Team:UC Chile2/Cyanolux/Project

From 2012.igem.org

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<p>The lux operon is a group of genes that are responsible for density-dependent bioluminescent behavior
<p>The lux operon is a group of genes that are responsible for density-dependent bioluminescent behavior
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in various prokariotic organisms such as <i>Vibrio fischeri</i> and <i>Photorabdus luminescens</i>. In <i>V. fischeri</i>, the operon is composed of 8 genes: LuxA and LuxB encode for the monomers of a heterodimeric luciferase; LuxC, LuxD and LuxE code for fatty acid reductases enzymes and LuxR and LuxI are responsible for the regulation of the whole operon.</p>
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in various prokariotic organisms such as <i>Vibrio fischeri</i> and <i>Photorabdus luminescens</i> [[#12|12]]. In <i>V. fischeri</i>, the operon is composed of 8 genes: LuxA and LuxB encode for the monomers of a heterodimeric luciferase; LuxC, LuxD and LuxE code for fatty acid reductases enzymes and LuxR and LuxI are responsible for the regulation of the whole operon [[#13|13]].</p>
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<p>Lastly LuxG is believed to act as a FMNH2 dependent FADH reductase, although luminescence is barely affected
<p>Lastly LuxG is believed to act as a FMNH2 dependent FADH reductase, although luminescence is barely affected
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in its absence. The n-decanal ( n= 9 to 14) substrate oxidization to n-decanoic acid by the LuxAB heterodimer is coupled with the reduction of FMNH to FMNH2 and the releasing of oxygen and x photons of light at x wavelength.</p>
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in its absence [[#14|14]]. The n-decanal ( n= 9 to 14) substrate oxidization to n-decanoic acid by the LuxAB heterodimer is coupled with the reduction of FMNH to FMNH2 and the releasing of oxygen and light.</p>
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<p>The carboxylic group of the product is then reduced to aldehyde by CDE proteins allowing the reaction to
<p>The carboxylic group of the product is then reduced to aldehyde by CDE proteins allowing the reaction to
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start over.</p>
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start over [[#15|15]].</p>
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<img src="http://2012.igem.org/wiki/images/5/56/Alagain_uc_chile.jpg" width="300" align="left" style ="margin-right:15px"></html>
<img src="http://2012.igem.org/wiki/images/5/56/Alagain_uc_chile.jpg" width="300" align="left" style ="margin-right:15px"></html>
<p>LuxAB genes have been widely used as reporters dependent on the addition of n-decanal to the culture
<p>LuxAB genes have been widely used as reporters dependent on the addition of n-decanal to the culture
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media and in 2010, the Cambridge iGEM team engineered LuxABCDEG to an <i>E. coli</i>-optimized biobrick
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media [[#16|16]] and in 2010, the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team] engineered LuxABCDEG to an <i>E. coli</i>-optimized biobrick
format, uncoupling it from the LuxR and LuxI regulation.</p>
format, uncoupling it from the LuxR and LuxI regulation.</p>
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(11)Kucho, K., Aoki, K., Itoh, S., & Ishiura, M., (2005). Improvement of the bioluminescence reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp. strain PCC 6803. Genes Genet. Syst.  80, p. 19–23
(11)Kucho, K., Aoki, K., Itoh, S., & Ishiura, M., (2005). Improvement of the bioluminescence reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp. strain PCC 6803. Genes Genet. Syst.  80, p. 19–23
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(12)Meighen, E. a. (1991). Molecular biology of bacterial bioluminescence. Microbiological reviews, 55(1), 123-42.
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(13)Dunlap, P. V. (1999). Quorum regulation of luminescence in Vibrio fischeri. Journal of molecular microbiology and biotechnology, 1(1), 5-12.
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(14)Luciferase, R. (2001). Differential Transfers of Reduced Flavin Cofactor and Product by Bacterial Flavin. Society, 1749-1754.
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(15) Kelly, C. J., Hsiung, C.-J., & Lajoie, C. a. (2003). Kinetic analysis of bacterial bioluminescence. Biotechnology and bioengineering, 81(3), 370-8. doi:10.1002/bit.10475
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(16)Tehrani, G. A., Mirzaahmadi, S., Bandehpour, M., & Laloei, F. (2011). Molecular cloning and expression of the luciferase coding genes of Vibrio fischeri. Journal of Biotechnology, 10(20), 4018-4023. doi:10.5897/AJB10.2363
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Latest revision as of 22:52, 25 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012