Team:UC Chile/Cyanolux/Results short

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We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer.  
We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer.  
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[[File: UC_Chile-decanalgraph.jpg]]
 
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While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This let us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.
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While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This led us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.
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During the Latin America Jamboree, we had a chat with a couple of judges and a student from the Universidad de los Andes, David Olarte, on how to induce Synechocystis with n-decanal.
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During the Latin America Jamboree, we had a chat with a couple of judges and a student from Universidad de los Andes, David Olarte, on how to induce Synechocystis with n-decanal.
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David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods we finally were able to see light emittion, confirming prescence of catalytically active luciferase.
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David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods we finally were able to see light emission, confirming presence of catalytically active luciferase. Thanks David!
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<h1>Experimental Highlights</h1>
<h1>Experimental Highlights</h1>
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Latest revision as of 04:01, 27 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012