Team:UC Chile/Cyanolux/Project short

From 2012.igem.org

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We designed two recombination plasmids backbones  One targets a gene essential for our chassis survival in the enviroment (link:see biosafety) and the other one a neutral site.
We designed two recombination plasmids backbones  One targets a gene essential for our chassis survival in the enviroment (link:see biosafety) and the other one a neutral site.
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[[File:UC_Chile-IntKstrategy | 400px | left]]
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[[File:UC_Chile-IntKstrategy.jpg | 400px | left]]
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[[File:UC_Chile-IntSstrategy | 400px | right]]
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[[File:UC_Chile-IntSstrategy.jpg | 400px | right]]
Our strategy was to insert the LuxAB genes (luciferase) under a circadian promoter with an expression peak right after dusk, in the neutral recombination plasmid; and the LuxCDEG genes (substrate production) with the appropriate promoter in the biosafety plasmid.
Our strategy was to insert the LuxAB genes (luciferase) under a circadian promoter with an expression peak right after dusk, in the neutral recombination plasmid; and the LuxCDEG genes (substrate production) with the appropriate promoter in the biosafety plasmid.

Revision as of 01:56, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012