# Team:UC Chile/Cyanolux/Future

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Promoters Redesigned

- + We are designing new versions of our chosen promoters. Now we have changed the promoter construction methodology in terms of the lenght of the upstream sequence considered. + Instead of considering just 150-200bp, up to 1000bp will be amplified from Synechocystis genome. + +

Transcriptional verification

+ Even if our promoters are o.k, colony PCRs can´t tell us wether the transcriptional machinery of our cyanobacteria is recognizing our constructs only  . + We are designig new primers to make  RT-PCRs that unmistakably verify transcription of the Lux genes  at the specified hours. + +