Team:UC Chile/Cyanolux/Future

From 2012.igem.org

(Difference between revisions)
Line 1: Line 1:
{{UC_Chile4}}
{{UC_Chile4}}
-
<html><a href="http://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp"><img src="http://2012.igem.org/wiki/images/a/ab/UC_Chile-Continue_button.jpg" align="right">
+
<h1>Promoters Redesigned</h1>
-
</html>
+
We are designing new versions of our chosen promoters. Now we have changed the promoter construction methodology in terms of the lenght of the upstream sequence considered.
 +
Instead of considering just 150-200bp, up to 1000bp will be amplified from Synechocystis genome.
 +
 
 +
<h1>Transcriptional verification</h1>
 +
Even if our promoters are o.k, colony PCRs can´t tell us wether the transcriptional machinery of our cyanobacteria is recognizing our constructs only  .
 +
We are designig new primers to make  RT-PCRs that unmistakably verify transcription of the Lux genes  at the specified hours.
 +
 
 +
<h1>Cyrcadian expression characterization</h1>
 +
We have allready built part [http://partsregistry.org/Part:BBa_K743018 BBa_K743018] to characterize differential expression of sfGFP at different moments of the day.
 +
Other promoters (new ones or those allready built) will be placed by means of Gibson assembly controlling the expression of this reporter.
 +
 
 +
<h1>New Biosafety strategies</h1>
 +
We have allready assembled part [http://partsregistry.org/Part:BBa_K743010 psb1C3_IntS] wich has a double goal: to insert LuxCDEG genes into Synechocystis and to confere a copper suceptibility that makes this cells unable to thrive in the enviroment.
 +
We are also designing primers to built our [http://2012.igem.org/File:UC_Chile-Animacion-mE-gen.gif new biosafety system ] based on mE genes and MgSO4, wich will be tested as soon as it is ready.
{{UC_Chilefooter}}
{{UC_Chilefooter}}

Revision as of 03:28, 27 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012