Team:UC Chile/Biosafety

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<h1>Susceptibility Construct</h1>
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<h1>Biosafety proposals</h1>
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<font size="4">WHY Biosafety? </font>
<font size="4">WHY Biosafety? </font>
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All people need to feel safe. Instinct drives behavior; therefore no technology will have a widespread use if safety is not guaranteed. This issue has been addressed by several research groups, however no Biosafe Biobrick standards have been defined within the iGEM community.
All people need to feel safe. Instinct drives behavior; therefore no technology will have a widespread use if safety is not guaranteed. This issue has been addressed by several research groups, however no Biosafe Biobrick standards have been defined within the iGEM community.
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We strongly believe that biosafety is not an option but a fundamental requirement regardless of the intrinsic risk of the DNA circuit.
We strongly believe that biosafety is not an option but a fundamental requirement regardless of the intrinsic risk of the DNA circuit.
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Under this vision, we have conceived a system inspired by nature, easy to use, cheap and extensible to all gram negative bacteria that we urge to be introduced mandatory to all plasmid backbones in the registry.  
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Under this vision, we have conceived Two main systems, one is inspired by nature, easy to use, cheap and extensible to all gram negative bacteria that -if working properly- we urge to be introduced mandatory to all plasmid backbones in the registry.  
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To make this vision a reality we propose the inclusion of a short and simple lysis gene under a constitutive promoter in all biobrick plasmids. This sequence codes for the mE gen phi X174 phage which inhibits peptidoglycan biosynthesis(Ref).  
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The second system is an integrative plasmid that knock out a gene necessary for copper stress tolerance.
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In this way it would kill any gram negative bacteria unless grown at all times under higher magnesium sulfate concentrations than those found in nature. In consequence, cells expressing the gen would be unable to thrive in an environment other than strict laboratory conditions.
 
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Description : Although the mechanism for which Mg+2 interferes with the mE gene is not known, when Mg+2 concentrations of 0.2M are present, the mE gene does not exert any deleterious effect on bacteria.
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<h2>Killer gene</h2>
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To our vision a reality we propose the inclusion of a short and simple lysis gene under a constitutive promoter in all biobrick plasmids. This sequence codes for the mE gen phi X174 phage which inhibits peptidoglycan biosynthesis(Ref).
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In this way it would kill any gram negative bacteria unless grown at all times under higher magnesium sulfate concentrations than those found in nature. In consequence, cells expressing the gen would be unable to thrive in an environment other than strict laboratory conditions.
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[[File:UC_Chile-Animacion-mE-gen.gif|center]]
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Description : Although the mechanism for which MgSO4 interferes with the mE gene is not known, when MgSO4 concentrations of 0.2M are present, the mE gene does not exert any deleterious effect on bacteria.
Our next goal is to test this biosafety mechanism by assembling a model construct to transform E. coli in order to characterize its effectiveness.
Our next goal is to test this biosafety mechanism by assembling a model construct to transform E. coli in order to characterize its effectiveness.
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<h2>Susceptibility Construct</h2>
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<html><center><img src="https://static.igem.org/mediawiki/2012/8/81/Suceptibyflv.jpg" align="left" width="720"></center></html>
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<a href="https://2012.igem.org/Team:UC_Chile/Community_outreach"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right">
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Latest revision as of 03:25, 27 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012