Team:UC Chile/Accomplishments

From 2012.igem.org

(Difference between revisions)
(Judging Criteria)
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<br>✓ At least one new submitted and well-characterized standard BioBrick Part or Device. A new application of and outstanding documentation (quantitative data showing the Part’s/ Device’s function) of a previously existing BioBrick part in the “Experience” section of that BioBrick’s Registry entry also counts.
<br>✓ At least one new submitted and well-characterized standard BioBrick Part or Device. A new application of and outstanding documentation (quantitative data showing the Part’s/ Device’s function) of a previously existing BioBrick part in the “Experience” section of that BioBrick’s Registry entry also counts.
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<i>We submitted parts [http://partsregistry.org/Part:BBa_K743000 BBa_K743000] and [http://partsregistry.org/Part:BBa_K743001 BBa_K743001] wich were verified by digestion and sequencing. The functioning of these parts was extensively proved by our succesfull transformation of synechocystis, what is well documented in our [http://2012.igem.org/Team:UC_Chile/Cyanolux/Results results].
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<i>We submitted parts [http://partsregistry.org/Part:BBa_K743000 BBa_K743000] and [http://partsregistry.org/Part:BBa_K743001 BBa_K743001] which were verified by digestion and sequencing. The functioning of these parts was extensively proved by our successful transformation of synechocystis that is well documented in our [http://2012.igem.org/Team:UC_Chile/Cyanolux/Results results].
We also characterized the functioning of KanR resistance genes [http://partsregistry.org/Part:BBa_P1003 P1003] and [http://partsregistry.org/Part:BBa_P1007 P1007] in Synechocystis PCC6803.
We also characterized the functioning of KanR resistance genes [http://partsregistry.org/Part:BBa_P1003 P1003] and [http://partsregistry.org/Part:BBa_P1007 P1007] in Synechocystis PCC6803.
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Lastly, we quantitatively characterized the [http://partsregistry.org/Part:BBa_K325909 Luxbrick] in different temperature, o.d, glucose concentration and growth fase conditions.</i>
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Lastly, we quantitatively characterized the [http://partsregistry.org/Part:BBa_K325909 Luxbrick] in different temperature, O.D, glucose concentration and growth fase conditions.</i>
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<i>Parts [http://partsregistry.org/Part:BBa_K743000 BBa_K743000] and [http://partsregistry.org/Part:BBa_K743001 BBa_K743001] worked as expected in Synechocystis double homologous recombination transformations.
<i>Parts [http://partsregistry.org/Part:BBa_K743000 BBa_K743000] and [http://partsregistry.org/Part:BBa_K743001 BBa_K743001] worked as expected in Synechocystis double homologous recombination transformations.
Part [http://partsregistry.org/Part:BBa_K743017 BBa_K743017] showed inducible expression as planned.
Part [http://partsregistry.org/Part:BBa_K743017 BBa_K743017] showed inducible expression as planned.
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Device [http://partsregistry.org/Part:BBa_K743006 BBa_K743006] showed the right results, i.e was usefull for transformation and selection in Synechocystis.</i>
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Device [http://partsregistry.org/Part:BBa_K743006 BBa_K743006] showed the right results, i.e was useful for transformation and selection in Synechocystis.</i>
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<br>✓Characterize the operation of at least one new BioBrick Part or Device and enter this information in the “Main Page” section of that Part’s/Device’s Registry entry
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<br>✓Characterize the operation of at least one new BioBrick Part or Device and enter this information in the “Main Page” section of that Part’s/Device’s Registry entry.
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<i>Device [http://partsregistry.org/Part:BBa_K743006 BBa_K743006] undergoes double homologous recombination in Synechocystis PCC6803 and conferes kanamycin resistance. Other sequences can be added to it and its effectiveness remains as shown in [http://partsregistry.org/Part:BBa_K743005 BBa_K743005]</i>
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<i>Device [http://partsregistry.org/Part:BBa_K743006 BBa_K743006] undergoes double homologous recombination in Synechocystis PCC6803 and confers kanamycin resistance. Other sequences can be added to it and its effectiveness remains as shown in [http://partsregistry.org/Part:BBa_K743005 BBa_K743005]</i>

Revision as of 05:52, 26 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012