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Team:UANL Mty-Mexico/Modeling/Biosensor
From 2012.igem.org
Biosensor
Our biosensor model is a continuation of the previously exposed transport and accumulation model with the addition of the production of luciferase as a function of ArsR|As concentration. In this way, we can relate the concentration of extracellular As to the production of luciferase.
ODEs
Parameters
Parameter table
|
Parameter |
Description |
Value |
References |
|
αmArsR |
Maximal transcription rate of ArsR |
3.74 nM/min |
Assumptions |
|
αmFluc |
Maximal transcription rate of Fluc |
1.40 nM/min |
Assumptions |
|
αpArsR |
Maximal translation rate of ArsR |
9.74 nM/min |
Assumptions |
|
αpFluc |
Maximal translation rate of Fluc |
2.06 nM/min |
Assumptions |
|
δmRNAArsR |
Degradation rate of ArsR mRNA |
2.16x10-1 min-1 |
Assumptions, GlpP, SubtiWiki. |
|
δmRNAFluc |
Degradation rate of Fluc mRNA |
3.37x10-2 min-1 |
Assumptions, Suter, et al., (2011), Karetnivok and Lehto (2006). |
|
δArsR |
Degradation rate of ArsR |
2.31x10-2 min-1 |
Assumptions |
|
δFluc |
Degradation rate of Fluc |
2.60x10-2 min-1 |
Assumptions, Auld, et al., (2008) |
|
proars |
Concentration of ars promoter |
aprox. 1*plasmid copy (nM) |
Assumptions |
Simulations
