Team:NTNU Trondheim/Protocols

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This is a list of recipes and protocols used by the team.

Contents

1 Transformation
2 Recipes
3 Growth media
4 Antibiotics

Transformation

Official iGEM transformation protocol: http://partsregistry.org/Help:Protocols/Transformation

Recipes

Growth media

LB-medium (LB-Lennox): 10 g tryptone 5 g yeast extract

Antibiotics

Ampicillin stock solutions: 100 mg/mL Kanamycin stock solutions: 100 mg/mL

Ampicillin media concentration: 100 ug/mL = 1 mL stock solution/L medium Kanamycin media concentration: 100 ug/mL = 1 mL stock solution/L medium

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