Team:NTNU Trondheim/Protocols
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{{:Team:NTNU_Trondheim/Templates/Header}} | {{:Team:NTNU_Trondheim/Templates/Header}} | ||
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=Protocols= | =Protocols= | ||
- | This is a list of recipes and protocols used by the team. | + | This is a list of recipes and protocols used by the team. |
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==Transformation== | ==Transformation== | ||
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Using a sterile toothpick, pick/scratch a single colony from the transformants. Drop the toothpick into a plastic tube with 3 mL sterile liquid medium with the appropriate antibiotic(s). Close the tube but leave the cap slightly open to allow oxygen to enter, and incuate at 37 C with shaking. | Using a sterile toothpick, pick/scratch a single colony from the transformants. Drop the toothpick into a plastic tube with 3 mL sterile liquid medium with the appropriate antibiotic(s). Close the tube but leave the cap slightly open to allow oxygen to enter, and incuate at 37 C with shaking. | ||
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==DNA Isolation== | ==DNA Isolation== | ||
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Concentrations of DNA after isolation was measured with the [http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf NanoDrop ND-1000 Spectrophotometer] | Concentrations of DNA after isolation was measured with the [http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf NanoDrop ND-1000 Spectrophotometer] | ||
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==Recipes== | ==Recipes== |
Revision as of 23:27, 27 June 2012