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Team:NTNU Trondheim/Protocols
From 2012.igem.org
(Difference between revisions)
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This is a list of recipes and protocols used by the team. | This is a list of recipes and protocols used by the team. | ||
| - | + | ==Transformation== | |
Official iGEM transformation protocol: http://partsregistry.org/Help:Protocols/Transformation | Official iGEM transformation protocol: http://partsregistry.org/Help:Protocols/Transformation | ||
| - | + | ==Recipes== | |
| - | ==Growth media== | + | ===Growth media=== |
LB-medium (LB-Lennox): | LB-medium (LB-Lennox): | ||
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| - | ==Antibiotics== | + | ===Antibiotics=== |
Ampicillin stock solutions: 100 mg/mL | Ampicillin stock solutions: 100 mg/mL | ||
Revision as of 11:09, 20 June 2012
This is a list of recipes and protocols used by the team.
Contents |
Transformation
Official iGEM transformation protocol: http://partsregistry.org/Help:Protocols/Transformation
Recipes
Growth media
LB-medium (LB-Lennox): 10 g tryptone 5 g yeast extract
Antibiotics
Ampicillin stock solutions: 100 mg/mL Kanamycin stock solutions: 100 mg/mL
Ampicillin media concentration: 100 ug/mL = 1 mL stock solution/L medium Kanamycin media concentration: 100 ug/mL = 1 mL stock solution/L medium
