Team:KAIST Korea/Notebook Labnote/2012 10

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Revision as of 21:46, 26 October 2012

KAIST Korea 2012 iGEM

Notebook : Labnote-October

Labnote

October

October 1st 2012

No Special Event!
Back to the Calendar
October 2nd 2012

No Special Event!
Back to the Calendar
October 3rd 2012

 Flip Flop

Bxb1 double transformants vector miniprep & PCR check
Results

2_0903Fig1

Discussions

Seemed to every colonies have appropriate vectors.
Back to the Calendar
October 4th 2012

No Special Event!
Back to the Calendar
October 5th 2012

No Special Event!
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October 6th 2012

No Special Event!
Back to the Calendar
October 7th 2012

No Special Event!
Back to the Calendar
October 8th 2012

No Special Event!
Back to the Calendar
October 9th 2012

No Special Event!
Back to the Calendar
October 10th 2012

 Flip Flop

Bxb1_GTG_pBAD single cut check with different enzyme
Single cut with SnaBI

Results

2_0910Fig1

No desired size(5.6kb) vector appeared.
Back to the Calendar
October 11th 2012

No Special Event!
Back to the Calendar
October 12th 2012

 Flip Flop

Bxb1_GTG_pBAD colony #4~7 inoculation, miniprep and single cut check Single cut with SnaBI
Single cut with SnaBI

Results

2_0912Fig1

Cloning completed
Back to the Calendar
October 13th 2012

No Special Event!
Back to the Calendar
October 14th 2012

No Special Event!
Back to the Calendar
October 15th 2012

 Flip Flop

BBa_C0060, BBa_C0061, BBa_C0062 transformation into TOP10
Templates for Auto-regulated FlipFlop project.

Back to the Calendar
October 16th 2012

 Flip Flop

BBa_C0060, BBa_C0061, BBa_C0062, bFMO inoculation and enzyme cut check
BBa_C0060, BBa_C0061, BBa_C0062: from colony, EcoRI single cut

  • BBa_C0060: 2859bp
  • BBa_C0061: 2688bp
  • BBa_C0062: 2826bp
  • bFMO: from cell stock, EcoRI single cut


Results

2_0916#Fig#1

Back to the Calendar
October 17th 2012

 Flip Flop

Template preparation for OE PCR

Promoter construct: 210bp

Results

2_0917Fig1

Promoter PCR failed

2_0917Fig2
Back to the Calendar
October 18th 2012

 Flip Flop

Template preparation for OE PCR
Results

2_0918Fig1

Terminator PCR failed
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October 19th 2012

 Flip Flop

Template preparation for OE PCR
Terminator: 179bp

Results

2_0919Fig1

→ Gel extracted
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October 20th 2012

 Flip Flop

Template preparation for OE PCR
Bxb1 Xis: 777bp

Results

2_0920Fig1

bFMO PCR w/ various condtions

2_0920Fig2



Bxb1_Xis-pGEM_B1 transfomation
Synthesized gene – Bxb1_Xis arrived and transformed into TOP10

Back to the Calendar
October 21st 2012

No Special Event!
Back to the Calendar
October 22nd 2012

 Flip Flop

Overlapping extension PCR
Results

2_0922Fig1

→ Gel extracted
Back to the Calendar
October 23rd 2012

 Flip Flop

Overlapping extension PCR-Nested PCR
Results

2_0923Fig1

Discussions

There was too little full construct nested. We think this is due to primer interfering by its LVA homology.


Overlapping extension PCR 2
Results

2_0923Fig2

Discussions

Bands were more intense than those of first trial.
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October 24th 2012

No Special Event!
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October 25th 2012

No Special Event!
Back to the Calendar
October 26th 2012

No Special Event!
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October 27th 2012

No Special Event!
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October 28th 2012

No Special Event!
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October 29th 2012

No Special Event!
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October 30th 2012

No Special Event!
Back to the Calendar
October 31st 2012

No Special Event!
Back to the Calendar


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