Team:Goettingen/week14-2

V07_30

• Experiment:
  In order to perform a further swimming assay new over night cultures of fliC (DH10B), fliC (Salmonella), motA, motB and yhjH in DH10B, BL21, DH5alpha and XL1 Blue were prepared.
  

V08_01

• Experiment:
  Since some problems with the growing of the cultures appeared new over night cultures of fliC (DH10B), fliC (Salmonella), motA, motB and yhjH in DH10B, BL21, DH5alpha and XL1 Blue were prepared.
  

V08_02

• Experiment:
  LB medium containing ampicillin was inoculated with the over night culture and shaken until an optical density of 0.4-0.6 was reached. Meanwhile ampicillin containing M9 agar plates were prepared and autoclaved Whatman Filter Papers were imbued with tryptone solution. Then the cultures were dropped on the agar at a distance of 1 cm to the paper. On each plate also colonies hosting the empty vector pUC18 were dropped in order to have a reference. Two drops were rather applied into the agar than onto as done before, because some paper predicted faster motility. Also this time each plate was prepared in duplicates.
  
• Observations & Results:
  Unfortunately, even after a week no swimming could be observed only growth. We assume that the medium might not provide adequate swimming conditions and therefore we plan to test more motile strains and alter the medium composition if necessary.

• Experiment:
  Since we recently received new E. coli strains that are more motile than our usually used laboratory stains new over night cultures of MG1655 and RP437 were prepared.