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| - | <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head>
| + | {{GoettingenHeader_deu|deu=Team:Goettingen/Focus Groups_deu|eng=Team:Goettingen/Focus Groups}} |
| - | <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
| + | __TOC__ |
| - | <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
| + | |
| - | <link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
| + | |
| - | <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
| + | |
| - | <link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&action=creativecommons" rel="meta">
| + | |
| - | <link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=rss">
| + | |
| - | <link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=atom">
| + | |
| | | | |
| | + | == Fokus Gruppe #1 - Selektion / (bakterielles) Schwimmen == |
| | + | <html><a href="https://static.igem.org/mediawiki/2012/4/48/Goett_taxtaskf_b.jpg"><img width="900" src="https://static.igem.org/mediawiki/2012/6/60/Goett_Team1_focus_t2.jpg"></a></html> |
| | | | |
| - | <body class="mediawiki ns-0 ltr page-Team_Goettingen"> | + | In dieser Gruppe dreht sich alles um die Entwicklung eines Selektionssystems, welches die Auslese der am schnellsten schwimmenden <i>E. coli</i> Klone ermöglicht. In einer intensiven Literaturrecherche sammelten wir Zusammensetzungen verschiedenster Schwimmagarmedien und Anleitungen für Schwimm-Assays und evaluierten diese hinsichtlich unseres Zieles. Generell können Medien sich auf die verschiedensten Weisen voneinander unterscheiden, aber in unserem Fall sind die Agarkonzentration und der Nährstoffgehalt am bedeutendsten. Die Agarkonzentration muss niedrig genug sein, um Schwimmen zu erlauben, aber dennoch hoch genug um eine halbfeste Struktur bilden zu können. |
| | | | |
| | + | Da die <i>E. coli</i> Zellen nur Schwimmen, wenn sich keine Nährstoffe mehr in ihrer unmittelbaren Umgebung befinden, sollte der Nährstoffgehalt so niedrig wie möglich sein ohne dass das Wachstum der <i>E. coli</i> Zellen bedeutend beeinträchtigt wird. Der niedrige Nährstoffgehalt ist außerdem notwendig, um zu untersuchen, ob eine gerichtete Taxis in Abhängigkeit unsere getesteten Moleküle stattfindet. |
| | | | |
| | + | Mithilfe aller dieser unterschiedlichen Experimente werden wir in der Lage sein ein Selektionssystem zu etablieren, welches schnell und einfach zu handhaben ist und zudem reproduktive Resultate liefert. |
| | | | |
| | | | |
| - | <!-- start content -->
| + | [[File:Goett_Coli_select.jpg|center]] |
| | + | <br><br> |
| | | | |
| | | | |
| | + | == Fokus Gruppe #2 - Verbesserung der Geschwindigkeit == |
| | | | |
| - | <style> | + | <html><a href="https://static.igem.org/mediawiki/2012/9/93/Goett_team2_PS.jpg"><img width="900" src="https://static.igem.org/mediawiki/2012/0/04/Gr2_Speedsquad_t.jpg"></a></html> |
| - | h1.firstHeading { display: none; }
| + | |
| | | | |
| - | p {text-align: justify;}
| + | In dieser Gruppe konzentrieren wir uns auf die Verbesserung der Schwimmfähigkeiten von <i>E. coli</i>. Um dies zu erreichen wählten wir ein Set von Genen, die in das Schwimmverhalten der E. colis involviert sein könnten. Durch die Verwendung verschiedener Promotoren können die Expressionslevel dieser Gene kontrolliert und die modifizierten Bakterien anschließend auf Schwimm-Agarplatten getestet werden. |
| | | | |
| - | a:link { color: #004080; text-decoration: none}
| + | Zu den gewählten Genen gehören flhDC, welchesden Masterregulator für Motilität und Chemotaxis verschlüsselt oder auch motB, welches Bestandteile des bakteriellen Flagellums codiert. Weiterhin verwenden wir für unsere Experimente unterschiedliche Stämme von <i>E. coli</i> um Unterschiede in der Beweglichkeit identifizieren zu können. |
| - | a:visited { color:##003090; text-decoration: none}
| + | |
| - | a:hover { color:#f29400; text-decoration: none}
| + | |
| - | a:active { color:#f29400; text-decoration: none}
| + | |
| | | | |
| - | #bodyContent { padding: 10px auto; width: 910px; margin: auto; clear: none; }
| + | [[File:Goett_Coli_speed.jpg]] |
| | + | <br><br> |
| | | | |
| - | table#team_members { text-align: justify; border: 0; }
| + | == Fokus Gruppe #3 - Chemorezeptoren == |
| - | table#team_members h2, table#team_members h3 { clear: both; }
| + | |
| | | | |
| | + | <html><a href="https://static.igem.org/mediawiki/2012/3/30/Goett_group3.jpg"><img width="900" src="https://static.igem.org/mediawiki/2012/2/21/Goett_group3_t.jpg"></a></html> |
| | | | |
| - | /*-----------------------------------------------------------------------------------------------*/
| |
| - | div.MenuBar ul li ul.DropDownMenu {
| |
| - | display: none; /* Hides all drop-down menus. */
| |
| | | | |
| - | }
| + | In dieser Gruppe liegt der Hauptfokus darauf den Aspartat-Rezeptor von <i>E. coli</i> unter Kontrolle verschieden starker konstitutiver Promotoren in Vektoren zu klonieren. Nachdem die Effekte der Überexpression des Chemotaxis-Rezeptors |
| - | /* | + | untersucht wurden, ist die nächste Herausforderung die Mutagenese dieses Proteins. Diese soll der Erstellung einer Chemorezeptor-Bibliothek dienen. |
| - | li:hover works in IE7 and FF2.
| + | |
| - | a:hover works in IE6 and FF2.
| + | |
| - | a:hover breaks li:hover in FF2.
| + | |
| - | */
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
| + | |
| - | display: none; /* Hides all side menus. */
| + | |
| - | }
| + | |
| - | /*------------------------------------------------------------------------------------- Menu Bar */
| + | |
| - | div.MenuBar {
| + | |
| - | font: Verdana;
| + | |
| - | height: 30px;
| + | |
| - | width: 910px;
| + | |
| - | /*width: 100%*/
| + | |
| - | margin: 0;
| + | |
| - | border-top: 0;
| + | |
| - | border-right: 0;
| + | |
| - | border-left: 0;
| + | |
| - | padding: 0;
| + | |
| - | background: #00446b;
| + | |
| | | | |
| - | }
| |
| - | div.MenuBar ul {
| |
| - | font: Verdana;
| |
| - | text-align: center;
| |
| - | list-style-type: none;
| |
| - | margin: 0 auto;
| |
| - | border: 0;
| |
| - | padding: 0;
| |
| - | background: #00446b;
| |
| - | }
| |
| - | div.MenuBar ul li {
| |
| - | font: Verdana;
| |
| - | display: block;
| |
| - | padding: 0;
| |
| - | margin: 0;
| |
| - | font-size: 1.3em;
| |
| - | float: left;
| |
| - | background: #00446b;
| |
| - | text-align: center;
| |
| - | width: 107px;
| |
| - | position: relative; /* Sets the positioning context for each drop-down menu. */
| |
| - | }
| |
| - |
| |
| - | div.MenuBar ul li a {
| |
| - | font: Verdana;
| |
| - | display: block;
| |
| - | background: #00446b;
| |
| - | height: 22px; /* Keep height + padding-top + padding-bottom sync with the menu bar height. */
| |
| - | color: white;
| |
| - | padding-top: 4px;
| |
| - | padding-bottom: 4px;
| |
| - | padding-left: 1em; /* Sets the left space between top-level items. */
| |
| - | padding-right: 1em; /* Sets the right space between top-level items. */
| |
| - | text-decoration: none;
| |
| - | }
| |
| - |
| |
| - | /*------------------------------------------------------------------------------ Drop-Down Menus */
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu {
| |
| - | display: block;
| |
| - | width: 10em; /* Drop-down menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | height: 1em;
| |
| - | padding: 1px; /* Sets the drop-down menu "effective border" width. */
| |
| - | position: absolute;
| |
| - | top: 28px; /* Places the drop-down menu under the menu bar.
| |
| - | Keep it sync with the menu bar height. */
| |
| - | left: 0; /* Aligns the drop-down menu to its top-level item. */
| |
| - | background-color: #A3C0E0; /* Selected item. */
| |
| - | color: #FFFFFF;
| |
| - |
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| |
| - | width: 10em; /* Keep it sync with the drop-down menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | height: 1em;
| |
| - | padding-left: 0;
| |
| - | padding-right: 0;
| |
| - | background-color: #A3C0E0; /* Selected item. */
| |
| - | color: #FFFFFF;
| |
| - | }
| |
| - | ul.DropDownMenu li a span {
| |
| - | display: block;
| |
| - | padding-left: 0.75em; /* Sets the left space of each drop-down menu item. */
| |
| - | padding-right: 0.25em; /* Sets the right space of each drop-down menu item. */
| |
| - | text-align: right; /* Aligns the >> symbol to the right. */
| |
| - | }
| |
| - | ul.DropDownMenu li a span span {
| |
| - | float: left; /* Aligns the text (back) to the left. */
| |
| - | font: 12px Verdana; /* Required for IE55. */
| |
| - | height: 20px;
| |
| - | color: #FFFFFF;
| |
| - | }
| |
| - | /*----------------------------------------------------------------------------------- Side Menus */
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| |
| - | display: block;
| |
| - | width: 11em; /* Side menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | padding: 1px; /* Sets the side menu "effective border" width. */
| |
| - | position: absolute;
| |
| - | top: -1px; /* Aligns the side menu to its drop-down menu item.
| |
| - | Keep it sync with the side menu "effective border" width. */
| |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| |
| - | width: 11em; /* Keep it sync with the side menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | font: 12px Verdana; /* Required for IE55. */
| |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | }
| |
| - | div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
| |
| - | padding-left: 1.5em; /* Sets the left space of each side menu item. */
| |
| - | padding-right: 0.5em; /* Sets the right space of each side menu item. */
| |
| - | text-align: left;
| |
| - | font: 12px Verdana; /* Required for IE55. */
| |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | }
| |
| - | /*----------------------------------------------------------------------------- Browser Specific */
| |
| - | * html div.MenuBar ul li a {
| |
| - | float: left; /* Required for IE55 and IE6.
| |
| - | Breaks O9.
| |
| - | Hidden (* html) from non-IE browsers. */
| |
| - | }
| |
| - | * html ul.DropDownMenu li a:hover {
| |
| - | cursor: hand; /* Required for IE55.
| |
| - | Hidden (* html) from non-IE browsers. */
| |
| - | }
| |
| - | ul.DropDownMenu li a:hover {
| |
| - | cursor: pointer; /* Required for IE6 and IE7.
| |
| - | Hidding it (* html) from non-IE browsers breaks IE7!
| |
| - | }
| |
| - | * html div.MenuBar a:hover {
| |
| - | text-decoration: none; /* Required for IE55 and IE6.
| |
| - | Hidden (* html) from non-IE browsers. */
| |
| - | }
| |
| - | * html div.MenuBar ul li table,
| |
| - | * html div.MenuBar ul li table td {
| |
| - | border: 0; /* Required for IE55 and IE6.
| |
| - | Hidden (* html) from non-IE browsers. */
| |
| - | }
| |
| - | /*------------------------------------------------------------------------------- Default Colors */
| |
| - | div.MenuBar {
| |
| - | background-color: Menu;
| |
| - | border-bottom: 1px solid ButtonShadow;
| |
| - | }
| |
| - | div.MenuBar a {
| |
| - | background-color: Menu; /* Top-level unselected items. */
| |
| - | color: MenuText;
| |
| - | }
| |
| - | div.MenuBar ul li:hover a,
| |
| - | div.MenuBar ul li a:hover {
| |
| - | color: #A3C0E0;
| |
| - | background-color: Highlight; /* Top-level selected item. */
| |
| - | color: HighlightText;
| |
| - | }
| |
| - | /*...............................................................................................*/
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu {
| |
| - | background-color: ButtonShadow; /* Sets the drop-down menu "effective border" color. */
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| |
| - | background-color: Menu; Drop-down menu unselected items.
| |
| - | Sets the drop-down menu "effective background" color. */
| |
| - | color: MenuText;
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
| |
| - | background-color: Highlight; /* Drop-down menu selected item. */
| |
| - | color: HighlightText;
| |
| - | }
| |
| - | /*...............................................................................................*/
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| |
| - | background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| |
| - | background-color: Menu; /* Side menu unselected items.
| |
| - | Sets the side menu "effective background" color. */
| |
| - | color: MenuText;
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| |
| - | background-color: Highlight; /* Side menu selected item. */
| |
| - | color: HighlightText;
| |
| - | }
| |
| - | /*-----------------------------------------------------------------------------------------------*/
| |
| - |
| |
| - | /*-------------------------------------------------------------------------------------- General */
| |
| - | body {
| |
| - | background: white;
| |
| - | color: black;
| |
| - | margin: 0;
| |
| - | border: 0;
| |
| - | padding: 0;
| |
| - | }
| |
| - |
| |
| - |
| |
| - | div.MenuBar {
| |
| - | font: 13px arial, helvetica, sans-serif;
| |
| - | }
| |
| - | div.MenuBar ul {
| |
| - | font: 13px arial, helvetica, sans-serif; /* Required for IE55. */
| |
| - | }
| |
| - | /*--------------------------------------------------------------------------------------- Colors */
| |
| - | div.MenuBar {
| |
| - | background-color: #0064C8; /* Selected item. */
| |
| - | color: #FFFFFF;
| |
| - | border-bottom: 1px solid ButtonShadow;
| |
| - | }
| |
| - | div.MenuBar a,
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a,
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| |
| - | background-color: #00446b; /* Selected item. */
| |
| - | color: #FFFFFF;
| |
| - | }
| |
| - | div.MenuBar ul li:hover a,
| |
| - | div.MenuBar ul li a:hover,
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| |
| - | background-color: #A3C0E0; /* Selected item. */
| |
| - | color: #FFFFFF;
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu,
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| |
| - | background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
| |
| - | }
| |
| - | /*--------------------------------------------------------------------------------------- Widths */
| |
| - | /*
| |
| - |
| |
| - | /*
| |
| - | Menu Bar 1
| |
| - | Drop-Down Menu #2
| |
| - | */
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4 li a {
| |
| - | width: 15.5em; /* Drop-down menu width. */
| |
| - | }
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
| |
| - | width: 14em; /* Drop-down menu width. */
| |
| - | }
| |
| - |
| |
| - | /*...............................................................................................*/
| |
| - | /*
| |
| - | Menu Bar 1
| |
| - | Drop-Down Menu #2
| |
| - | Side Menu #1
| |
| - | */
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
| |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width. */
| |
| - | }
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
| |
| - | width: 15em; /* Side menu width. */
| |
| - | }
| |
| - | /*...............................................................................................*/
| |
| - | /*
| |
| - | Menu Bar 1
| |
| - | Drop-Down Menu #2
| |
| - | Side Menu #2
| |
| - | */
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 {
| |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width. */
| |
| - | }
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
| |
| - | width: 7em; /* Side menu width. */
| |
| - | }
| |
| - | /*...............................................................................................*/
| |
| - | /*
| |
| - | Menu Bar 1
| |
| - | Drop-Down Menu #2
| |
| - | Side Menu #3
| |
| - | */
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| - | <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Newspaper"><span><span><div style="text-indent:20px;">⋅ Newspaper</div></span></span></a></li>
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| - | Sprache: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> <a href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen/Focus_Groups">Englisch</a>, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> Deutsch <br>
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| - | <table id="toc" class="toc"><tbody><tr><td><div id="toctitle"><h2>Inhalte</h2> <span class="toctoggle"><a href="javascript:toggleToc()" class="internal" id="togglelink"></a></span></div>
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| - | <li class="toclevel-1"><a href="#Focus_Groups"><span class="tocnumber">1</span> <span class="toctext">Fokus Gruppen</span></a></li>
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| - | <li class="toclevel-1"> <div style="text-indent:10px;"><a href="#Focus_Groups#1"><span class="tocnumber"></span> <span class="toctext">#1 - Selektion / (bakterielles) Schwimmen </span></a></li>
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| - | <li class="toclevel-1"> <div style="text-indent:10px;"><a href="#Focus_Groups#2"><span class="tocnumber"></span> <span class="toctext">#2 - Verbesserung der Geschwindigkeit </span></a></li>
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| - | <li class="toclevel-1"> <div style="text-indent:10px;"><a href="#Focus_Groups#3"><span class="tocnumber"></span> <span class="toctext">#3 - Chemorezeptoren </span></a></li>
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| - | <h2><b><a name="Focus_Groups"></a>Fokus Gruppen</b></h2>
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| - | <h2><b><a name="Focus_Groups#1"></a>#1 - Selektion / (bakterielles) Schwimmen </b></h2>
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| - | <p align="justify" style="line-height:1.6em">
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| - | In dieser Gruppe dreht sich alles um die Entwicklung eines Selektionssystems, welches die Auslese der am schnellsten schwimmenden <i>E. coli</i> Klone ermöglicht. In einer intensiven Literaturrecherche sammelten wir Zusammensetzungen verschiedenster Schwimmagarmedien und Anleitungen für Schwimm-Assays und evaluierten diese hinsichtlich unseres Zieles. Generell können Medien sich auf die verschiedensten Weisen voneinander unterscheiden, aber in unserem Fall sind die Agarkonzentration und der Nährstoffgehalt am bedeutendsten. Die Agarkonzentration muss niedrig genug sein, um Schwimmen zu erlauben, aber dennoch hoch genug um eine halbfeste Struktur bilden zu können.
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| - | Da die <i>E. coli</i> Zellen nur Schwimmen, wenn sich keine Nährstoffe mehr in ihrer unmittelbaren Umgebung befinden, sollte der Nährstoffgehalt so niedrig wie möglich sein ohne dass das Wachstum der <i>E. coli</i> Zellen bedeutend beeinträchtigt wird. Der niedrige Nährstoffgehalt ist außerdem notwendig, um zu untersuchen, ob eine gerichtete Taxis in Abhängigkeit unsere getesteten Moleküle stattfindet.
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| - | <br>
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| - | Mithilfe aller dieser unterschiedlichen Experimente werden wir in der Lage sein ein Selektionssystem zu etablieren, welches schnell und einfach zu handhaben ist und zudem reproduktive Resultate liefert.
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| - | <h2><b><a name="Focus_Groups#2"></a>#2 - Verbesserung der Geschwindigkeit </b></h2>
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| - | In dieser Gruppe konzentrieren wir uns auf die Verbesserung der Schwimmfähigkeiten von <i>E. coli</i>. Um dies zu erreichen wählten wir ein Set von Genen, die in das Schwimmverhalten der E. colis involviert sein könnten. Durch die Verwendung verschiedener Promotoren können die Expressionslevel dieser Gene kontrolliert und die modifizierten Bakterien anschließend auf Schwimm-Agarplatten getestet werden.
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| - | Zu den gewählten Genen gehören flhDC, welchesden Masterregulator für Motilität und Chemotaxis verschlüsselt oder auch motB, welches Bestandteile des bakteriellen Flagellums codiert. Weiterhin verwenden wir für unsere Experimente unterschiedliche Stämme von <i>E. coli</i> um Unterschiede in der Beweglichkeit identifizieren zu können.
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| - | <h2><b><a name="Focus_Groups#3"></a>#3 - Chemorezeptoren</b></h2>
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| - | In dieser Gruppe liegt der Hauptfokus darauf den Aspartat-Rezeptor von <i>E. coli</i> unter Kontrolle verschieden starker konstitutiver Promotoren in Vektoren zu klonieren. Nachdem die Effekte der Überexpression des Chemotaxis-Rezeptors
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| - | untersucht wurden, ist die nächste Herausforderung die Mutagenese dieses Proteins. Diese soll der Erstellung einer Chemorezeptor-Bibliothek dienen.
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| | Idealerweise wird durch die Mutation der für chemisches Binden bedeutenden Liganden-Bindestelle die Spezifität des Rezeptors verändert. | | Idealerweise wird durch die Mutation der für chemisches Binden bedeutenden Liganden-Bindestelle die Spezifität des Rezeptors verändert. |
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In dieser Gruppe dreht sich alles um die Entwicklung eines Selektionssystems, welches die Auslese der am schnellsten schwimmenden E. coli Klone ermöglicht. In einer intensiven Literaturrecherche sammelten wir Zusammensetzungen verschiedenster Schwimmagarmedien und Anleitungen für Schwimm-Assays und evaluierten diese hinsichtlich unseres Zieles. Generell können Medien sich auf die verschiedensten Weisen voneinander unterscheiden, aber in unserem Fall sind die Agarkonzentration und der Nährstoffgehalt am bedeutendsten. Die Agarkonzentration muss niedrig genug sein, um Schwimmen zu erlauben, aber dennoch hoch genug um eine halbfeste Struktur bilden zu können.
Mithilfe aller dieser unterschiedlichen Experimente werden wir in der Lage sein ein Selektionssystem zu etablieren, welches schnell und einfach zu handhaben ist und zudem reproduktive Resultate liefert.
In dieser Gruppe konzentrieren wir uns auf die Verbesserung der Schwimmfähigkeiten von E. coli. Um dies zu erreichen wählten wir ein Set von Genen, die in das Schwimmverhalten der E. colis involviert sein könnten. Durch die Verwendung verschiedener Promotoren können die Expressionslevel dieser Gene kontrolliert und die modifizierten Bakterien anschließend auf Schwimm-Agarplatten getestet werden.
Zu den gewählten Genen gehören flhDC, welchesden Masterregulator für Motilität und Chemotaxis verschlüsselt oder auch motB, welches Bestandteile des bakteriellen Flagellums codiert. Weiterhin verwenden wir für unsere Experimente unterschiedliche Stämme von E. coli um Unterschiede in der Beweglichkeit identifizieren zu können.
Idealerweise wird durch die Mutation der für chemisches Binden bedeutenden Liganden-Bindestelle die Spezifität des Rezeptors verändert.
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