"

From 2012.igem.org

Weeks

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

Week 7: 23rd July - 29th July

Monday, 23rd June

Cloning:

4 different clones pCS2+ RFP => incubation in LB medium overnight at 37 degree celsius.

PCR:

PCR of pCS2+:

Reactants	Volumes (µl)
GC Buffer	10
dNTPs	1
DNA	1
H2O	32,5
Primers (FW and RV)	2,5 each
fusion polymerase	0,5

Tuesday, 24th June

Thursday, 26th June

Friday, 27th June