Team:EPF-Lausanne/Notebook/12 July 2012

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Revision as of 07:44, 13 July 2012 by Diego.marcos (Talk | contribs)




Maxiprep

2 maxipreps using 200 ml of LB each, for RO + Spc. and LovTAP + Amp.

In the morning we realized that the bacteria had been overnight at 30.7ºC, so we left them 4 extra hours at 37ºC.

  • Centrifuge them for 20 min at 4000 rpm, thow away the supernatant and continue with the Maxiprep protocol.
  • In the last step, centrifuge the Falcon tubes at 4000 rpm, for one hour.
  • Bring the tubes to hood in the cell lab.
  • Remove the supernatant with the vacuum pump.
  • Add 5 ml of ethanol, shake gently, and remove with the vacuum pump. Be careful with the DNA pellet!
  • Leave the tubes open, at the back of the hood, with the label "DNA drying".


Protocol: None

Forgot to insert protocol.


Comments

Insert comments about what happened.