Maxiprep

2 maxipreps using 200 ml of LB each, for RO + Spc. and LovTAP + Amp.

In the morning we realized that the bacteria had been overnight at 30.7ºC, so we left them 4 extra hours at 37ºC.

• Centrifuge them for 20 min at 4000 rpm, thow away the supernatant and continue with the Maxiprep protocol.
• In the last step, centrifuge the Falcon tubes at 4000 rpm, for one hour.
• Bring the tubes to hood in the cell lab.
• Remove the supernatant with the vacuum pump.
• Add 5 ml of ethanol, shake gently, and remove with the vacuum pump. Be careful with the DNA pellet!
• Leave the tubes open, at the back of the hood, with the label "DNA drying".