Team:Columbia-Cooper-NYC/Electroporation

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Revision as of 04:22, 3 October 2012


Electroporation Protocol

  • The following is the electroporation protocol provided by bioline:
  1. Place cuvettes and competent cells into ice
  2. Add 1µl of DNA to 50µl of competent cells into 1mL cuvettes as quickly as possible
  3. Keep cuvettes in ice until electroporation
  4. Electroporate cells at 1800V
  5. Place bacteria back into tube and add 100-200µl of LB
  6. Place samples in shaker for 37C for 1 hour

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