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Team:Chalmers-Gothenburg/Results
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=='''Results Summary'''== | =='''Results Summary'''== | ||
| - | Spot test analysis and growth measurements of yeast in urine were performed in order to test the survival of yeast cells in urinary medium. These initial experiments showed that yeast cells are able to survive in urinary medium which is an important prerequisite for the biosensor. | + | <!--Spot test analysis and growth measurements of yeast in urine were performed in order to test the survival of yeast cells in urinary medium. These initial experiments showed that yeast cells are able to survive in urinary medium which is an important prerequisite for the biosensor. |
The gene CWP2, encoding a mannoprotein in the cell wall was successfully deleted in the yeast strain IMFD-73. The deletion was confirmed by verification PCR. In addition, a lyticase assay showed that [IMFD-73 Δ''cwp2''::kanMX] was degraded faster than IMFD-73 and this leads us to the conclusion that the cell wall is somewhat weakened in the strains with the deleted cell wall mannoprotein CWP2. | The gene CWP2, encoding a mannoprotein in the cell wall was successfully deleted in the yeast strain IMFD-73. The deletion was confirmed by verification PCR. In addition, a lyticase assay showed that [IMFD-73 Δ''cwp2''::kanMX] was degraded faster than IMFD-73 and this leads us to the conclusion that the cell wall is somewhat weakened in the strains with the deleted cell wall mannoprotein CWP2. | ||
Revision as of 18:00, 13 September 2012
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