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Team:Chalmers-Gothenburg/Results
From 2012.igem.org
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Results Summary
Survival of yeast in urine
An initial test was performed in order to test the survival of yeast cells in urine, which is an important property of our biosensor if it should function as a pregnancy test. Pre-cultures of a normal lab yeast strain were prepared in 5ml YPD and let grown O/N at 30 °C. The day after, the cells were centrifuged, resuspended in 40 ml YPD and 40 ml filter sterilized urine respectively and grown in shake flasks at 37°C. OD600 measurements were taken at different points of time during the day, and the values can be seen in Figure 1. The cells in urine did not grow; the OD remained the same during the whole day, while the cells in YPD grew very well.
In order to test if the cells still are alive in the urine medium, 300 µl of each culture (after 4h) were taken and OD was adjusted to 0.5. The samples were diluted 3x, 9x, 27x and 81x and 10 µl aliquots of each dilution were spotted on an YPD plate (Figure 7). The cells from both the YPD and the urine media could grow, this means that the cells survived 4 h in urine and were still alive when we spotted them on the YPD plates even if they were unable to bud in the urine medium. Summarizing, the cells survive in urine but cannot grow.
Expression of human LH/CG receptor
Introduction of indigo synthesizing genes
Deletion of CWP2 gene
