Team:NTU-Taida/Human Practice/Information-Platform

From 2012.igem.org

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Facebook PAGES and Blog
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In order to let more people know about synthetic biology and the competition of iGEM, We not only held several campaigns between other Taiwanese iGEM teams, we also established a Blog ([http://igemntuadmin.blogspot.tw/]) and a Facebook page (http://www.facebook.com/NationalTaiwanUniversityiGEM) for publics around the world. All Facebook(FB) users have free access to this page. This is one of the attempts We made to promote the visibility of synthetic biology during general public and students of all ages. In order to increase accessibility, we translated the journal article to traditional Chinese. All the articles were posted in traditional Chinese, too. We summited articles about latest advances in synthetic biology and fields relating to iGEM, in a routine schedule, usually two to three articles per week. In sum, we had posted more than 30 articles form July, 2012. The content not only includes latest journal articles sharing, our iGEM NTU_Taida project, the detail process of how we establish our team and the cooperating meetings between us and iGEM team NYMU_Taipei, NCTU_Formosa ,two teams from Taiwan and another team in USA, UIUC-Illinois.
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==Modified P<sub>CI</sub>==
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<p style="text-indent: 2em;">The circuit incorporates a temperature sensitive cI promoter(CIts) to sense the temperature upshift. In our test we use a thermal adjustable plate reader to detect the mRFP flurorescence. In the beginning, we keep the temperature under 30 Celsius degrees for over 1 hour, and then detect the emission of mRFP. As the dimerized CIts repressor in lower temperature would specifically bind and repress P<sub>CI</sub>, and further hinder the expression of mRFP. We can expect the emission to be low under 610 nm wavelength. We then abruptly increase our temperature to 37 celsius degrees, as we expect the CIts dimer would decompose and lose the function of repressing mRFP expression. </p>
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The result was totally better than what we ever expected, we had 271 likes from FB users, and the number are still increasing! These users have more than 84,715 friends on FB, once they pushed the like button, these are all the potential audiences who can see NTU_Taida articles. We also got several followers who chose to receive FB notices if we release any new article. Each time we released a new article, you can see that more than 200 FB users will see it within a day. Some undergraduate students even came to us, face to face, to know more . They wanted to start their own iGEM team early, to think of a great project, and recruit their members early. We gave them our best wishes and promised to share our experiences in iGEM competition.
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[[File:NTU-Taida-Result-Thermal-pCI.png|600px|center]]
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Seeing all these great results, we want our internet platform to be more inspiring for any person who is interested. We start to collect any information about synthetic biology in Taiwan. Recently, we released a notice about symposiums , such  as the 6th IECA Conference 2012: Synthetic Biology with Applications to Biotechnology in National Taiwan university. We want this platform to be a space where all those who are interested in synthetic biology can share and get new information, not only now, but also in the future.
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<p style="text-indent: 2em;">The result showed a low level of mRFP expression under wavelength of 580 nm (excitation) and 610 nm (emission). After the sudden temperature upshift, the expression of mRFP steadily rises, and results in 5.7 folds increase in the 8th hour after the temperature upshift. </p>
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<p style="text-indent: 2em;">This proved the fact that repressor CIts and P<sub>CI</sub> can largely lead to increase in protein expression, and can be used in our circuit design as it may turn on the circuit inside human body and spontaneously close down after the bacteria is expelled out. </p>
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Revision as of 13:43, 26 October 2012

Information Platform

Human Practice: Information Platform


Contents

Modified PCI

The circuit incorporates a temperature sensitive cI promoter(CIts) to sense the temperature upshift. In our test we use a thermal adjustable plate reader to detect the mRFP flurorescence. In the beginning, we keep the temperature under 30 Celsius degrees for over 1 hour, and then detect the emission of mRFP. As the dimerized CIts repressor in lower temperature would specifically bind and repress PCI, and further hinder the expression of mRFP. We can expect the emission to be low under 610 nm wavelength. We then abruptly increase our temperature to 37 celsius degrees, as we expect the CIts dimer would decompose and lose the function of repressing mRFP expression.

NTU-Taida-Result-Thermal-pCI.png


The result showed a low level of mRFP expression under wavelength of 580 nm (excitation) and 610 nm (emission). After the sudden temperature upshift, the expression of mRFP steadily rises, and results in 5.7 folds increase in the 8th hour after the temperature upshift.

This proved the fact that repressor CIts and PCI can largely lead to increase in protein expression, and can be used in our circuit design as it may turn on the circuit inside human body and spontaneously close down after the bacteria is expelled out.