Team:EPF-Lausanne/Notebook/30 May 2012

From 2012.igem.org



Observation of transformed colonies

Amp plates
  • Negative control plates almost empty (OK)
  • Both experimental plates have colonies (OK)
Spc plates
  • Negative control plates has several very small colonies (OK)
  • Both experimental plates have small things that look like dew drops (but don't move) (not sure if OK)


Prepared LB medium for next steps



Protocol: Preparation of LB Medium

Put 25g of powder in 400ml + add ~100ml to complete to 500ml. Put in autoclave (don't forget to loosen the caps!).