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| | <html><a name="scroll-one"></a></html>'''Characterization Data''' | | <html><a name="scroll-one"></a></html>'''Characterization Data''' |
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| - | A current problem with designing synthetic networks is a lack of ability to externally control the system. By integrating and optimizing bacterial systems in ''S. cerevisiae'' the uOttawa team hopes to generate a new inducible system that can be used for synthetic gene network design in yeast.
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| - | '''Combinatorial Mating and Gene Regulatory Systems'''
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| - | An advantage of using yeast as a model organism is its ability to exist in both haploid and diploid states. The uOttawa team plans to utilize this ability to combinatorially mate haploid strains to build gene networks. A haploid
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| - | strain that acts as reporter for gene regulatory proteins can be used as a "testing" strain and mated with other haploid "sample" strains to quickly and efficiently test gene regulatory functions.
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| - | '''Shuttle Vector'''
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| - | By building an ''E.coli/S.cerevisiae'' shuttle vector we can take advantage of the high reproductive rate
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| - | of ''E.coli'' and the gene synthesis capabilities of ''S.cerevisiae''. Networks will be built via homologous recombination in yeast and replicated in bacteria. Traditional drug selection will be supplemented with colour selection to increase the accuracy of the transformations.
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Revision as of 00:05, 4 October 2012
Results 1 Results 2
Characterization Data
Combinatorial Mating and Gene Regulatory Systems
An advantage of using yeast as a model organism is its ability to exist in both haploid and diploid states. The uOttawa team plans to utilize this ability to combinatorially mate haploid strains to build gene networks. A haploid
strain that acts as reporter for gene regulatory proteins can be used as a "testing" strain and mated with other haploid "sample" strains to quickly and efficiently test gene regulatory functions.
Shuttle Vector
By building an E.coli/S.cerevisiae shuttle vector we can take advantage of the high reproductive rate
of E.coli and the gene synthesis capabilities of S.cerevisiae. Networks will be built via homologous recombination in yeast and replicated in bacteria. Traditional drug selection will be supplemented with colour selection to increase the accuracy of the transformations.
"
