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Team:Wageningen UR/Parts
From 2012.igem.org
Contents |
Parts
In the Open Access scientific community that iGEM is, the Registry of Standard Parts plays a crucial role. As teams construct new parts and devices, they make them available to anyone who might want to use, add or improve them. However, the current registry is not only hard to navigate (see The Constructor), it also turns out to contain non-functioning and even non-existent parts. To work towards a better Open Source future, we think it is of great importance that apart from quantity, the quality of the registry should improve as well. It is up to all users to make this happen. We tried to contribute to the quality of the registry not only by sending in our bricks (which were all sequenced), but also by validating other bricks and improving some[1, 2].
Parts we made
<groupparts>iGEM012 Wageningen_UR</groupparts>
Parts we used
For our project, we used few bricks from the 2012 Spring Distribution Kit. A list is included below.
| Part | Name | Description |
| BBa_B0015 | Terminator | double terminator (B0010-B0012) |
| BBa_I13522 | pTet GFP | Untagged GFP behind a constitutive promoter. |
| BBa_J04450 | RFP Coding Device | RFP Coding Device (iptg + Cap induced, pSB1C3 backbone) |
| BBa_J04500 | IPTG inducable promotor with RBS | R0010.B0034 |
| BBa_K197038 | BBb - BseRI/AcuI entry vector | This part is and entry vector in BBb Format. It is flanked by BamHI, BseRI, BglII, and AcuI sites and is used in the 2AB Layered Assembly scheme developed at UC Berkeley. |
Parts we ordered
For our project, we ordered few bricks from the iGEM Registry. A list is included below.
| Part | Name | Description |
| BBa_K197021 | EILD | One peptide of a heterodimeric leucine zipper that dimerizes with KILR. |
| BBa_K197022 | KILR | A heterodimeric peptide that dimerizes to EILD. This can be used for adhering two cells together to evolve new two cell chemistry and functions. |
| BBa_J3901 | PrFe-mRFP1 | This device coupled the promoters PI and PII of rus operon from Acidithiobacillus ferrooxidans with a monomeric red fluorescent protein (BBa_E1010) used as a signal, showing specific sensorbility response to iron ions presence. |
| BBa_I765010 | Iron promoter -EYFP reporter | Iron promoter expresion is reported by the trascription of EYFP reporter. |
Parts we standardized
Two of these parts (BBa_K197021 and BBa_K197022) were in a non-standard backbone and format. For both parts, we tried to brick them by adding standard pre- and suffixes and inserting them in the pSB1C3 standard backbone. Unfortunately, this only worked for one of the parts. We uploaded the standardized version of BBa_K197022 on the registry as BBa_K883600.
Faulty parts
Every year an incredible amount of biobricks is added to the part registry. Since they come in such great numbers, it is an impossible task to validate them all. The use of unvalidated bricks comes with a risk, which we had to take. It is unfortunate to find out that a particular brick is non-functional, but it also gives us the opportunity to improve the quality of the parts registry as a whole. Two of the ordered bricks were not in a standard backbone nor format, so we tried to fix that. Two other parts showed to be not as expected, so we documented that.
Two of these parts (BBa_K197021 and BBa_K197022) were in a non-standard backbone and format. For both parts, we tried to brick them by adding standard pre- and suffixes and inserting them in the pSB1C3 standard backbone. Unfortunately, this only worked for one of the parts. We uploaded the standardized version of BBa_K197022 on the registry as BBa_K883600.
