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From 2012.igem.org

Team Achievements

All the work as described in the labjournals was executed by team members of iGEM Wageningen_UR 2012. The table below highlights some of the special achievements of this team. A detailed description of the other work done can be found in the Journal.

Activity	Done by	Team member?	requirement for
Measuring VLP size using DLS	Mark	Yes
Purification of VLPs using FPLC	Mark	Yes
Visualizing and measuring VLP size using EM	Mark, Wouter, Hugo, Kees	Yes
Construction of inside and outside modified VLPs	Hugo, Jeroen, Robert	Yes
Production of VLPs	All team members	Yes
Writing the protocol for HepBcAg VLPs	Kees	Yes
Design and assembly of GFP-E-coil construct	Lisa, Kees, Robert	Yes
Modification of existing BioBricks	Lisa, Robert	Yes
Teaching about synthetic Biology on a secondary school	Joël, Niels	Yes
Organizing the participation of iGEM in the Discovery Festival	Wouter and representatives of other Dutch iGEM teams	Partly
Modeling of subunits and VLP formation	Agneya, Thijs, Kees	Yes
The Constructor	Thijs, Jasper, iGEM Wageningen UR 2011	Partly
Isolation of the PLRV coat protein from natural samples	Marnix	Yes
Presenting a poster at a scientific conference	All team members	Yes
Organised a mini symposium in the beginning of the competition for all dutch teams	Jeroen	Yes
Interviewed stakeholders about our project	Nick, Kees, Jeroen	Yes

Table 1. Overview of attributions and achievements.

Acknowledgements

With special thanks to:

Our supervisors Dr. Mark van Passel and Floor Hugenholtz, for asking the right questions and advising in problem solving

Dr. Richard Kormelink, for providing the templates of CCMV and advising in VLP assembly

Dr. Afshin Mehraban, for practical advice in working with VLPs

Prof. Dr. George Lomonossoff and Dr. Hadrien Peyret for providing the HBcAg gene

Dr. Jan van Lent, for giving team members a crash course in electron microscopy

Dr. Servé Kengen, for assistance and explanation about the FPLC

Ing. Remko Fokking, for explanation about DLS

The whole staff of Systems and Synthetic biology, Wageningen UR, for the lab space and tolerating our presence.

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