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| | =Experimental Data= | | =Experimental Data= |
| | <hr/> | | <hr/> |
| - |
| |
| - | ==Abstract==
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| - |
| |
| - | ==Vectordesign==
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Project/Vector_Design"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ==Regulation of genexpression==
| |
| - | 50-100 words introduction
| |
| - |
| |
| - | ===Constituvie promoters===
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Project/Constitutive_Promoter"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ===Alcohol-inducible promoter===
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Project/Ethanol_Inducible_Promoter"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ===Light-switchable promoter===
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Project_Light_Switchable_Promoter"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ==Biosynthesis pathways==
| |
| - | 50-100 words introduction
| |
| - |
| |
| - | ===Limonene===
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Project_Limonene"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ===Thaumatine===
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Project/Thaumatin><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ===Caffeine===
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Project/Caffeine"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ===Xantohumol===
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Project/Xanthohumol"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ==Genome integration==
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Project/Genome_Integration"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ==Brewing our SynBio Beer==
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Project/Brewing"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ==Businessplan for our TUM Brew==
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Human Practice/Public"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | ==Our RFC for Standardization of BioBrick part descriptions==
| |
| - | <div class="bezel mfull">
| |
| - | 50 - 70 words introduction
| |
| - | <br><hr>
| |
| - | [[file:TUM12_experiment_overwiew.png|500px|thumb|right| Explanations on the figure]]
| |
| - | <center>'''Experimental results:'''</center><br>
| |
| - | 50-100 words on results
| |
| - |
| |
| - | <center>'''Conclusion and outlook:'''</center><br>
| |
| - |
| |
| - | <div class="noborder" style="float:left">
| |
| - | <html><a href="https://2012.igem.org/Team:TU_Munich/Results/RFC"><img width="200px" src="https://static.igem.org/mediawiki/2012/3/33/TUM12_readmore.png"/></a></html>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - |
| |
| - | == References ==
| |
| - | <div class="bezel mfull">
| |
| - | * [1] Synthesis and processing of the plant protein thaumatin in yeast, Luppo Edens, Isaäc Bom, Adrianus M. Ledeboer, Jan Maat, Marjolein Y. Toonen, Chris Visser, C. Theo Verrips. Cell Volume 37, Issue 2, June 1984, Pages 629–633
| |
| - | * [2] C.L Miranda, J.F Stevens, V Ivanov, M McCall, B Frei, M.L Deinzer, D.R Buhler, Antioxidant and prooxidant actions of prenylated and nonprenylated chalcones and flavanones in vitro, J. Agric. Food Chem., 48 (2000), pp. 3876–3884
| |
| - | * [3] Insertion of Coffein, Römpp Online. Version 3.19
| |
| - | * [4] Use of the KlADH4 Promoter for Ethanol-Dependent Production of Recombinant Human Serum Albumin in Kluyveromyces lactis. Michele Saliola, Cristina Mazzoni, Nicola Solimando, Alessandra Crisà, Claudio Falcone, Gerard Jung, and Reinhard Fleer. Appl Environ Microbiol. 1999 January; 65(1): 53–60.
| |
| - | * [5] Development of a genetic switch based on a photosensitive protein signals the beginning of light-based biological engineering. Andrew R. Mendelsohn Nature Biotechnology, Vol. 20, October 2002, Pages 985-987
| |
| - | * [6] Molecular analysis of UAS, a cis element cotaining stress response elements responsible for ethanol induction in the KlADH4 of Kluyveromyces lactis. Cristina Mazzoni, Francesca Santori, Michele Saliola and Claudio Falcone Research in Microbiology, Vol. 151, 2000, Pages 19-28
| |
| - |
| |
| - | </div>
| |
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