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Team:TU Darmstadt/Materials/tctB162
From 2012.igem.org
(Difference between revisions)
(→tctB162 Pst1) |
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rev: gtttcttc'''ctgcagcggccgctactagta'''ttagccagcgatgaagctaggcc | rev: gtttcttc'''ctgcagcggccgctactagta'''ttagccagcgatgaagctaggcc | ||
=== tctB162 Pst1 === | === tctB162 Pst1 === | ||
| - | [https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for the elimination of a Pst1 site inside the tctB162 BioBrick. | + | [https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for the elimination of a [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI Pst1] site inside the tctB162 BioBrick. |
5' 3' | 5' 3' | ||
fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC | fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC | ||
rev: GGCCACACGGGGAACTGGAGATTCAGCGCC | rev: GGCCACACGGGGAACTGGAGATTCAGCGCC | ||
| + | |||
=== tctB162 RBS === | === tctB162 RBS === | ||
[https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for the insertion of a RBS based on the sequence of [http://partsregistry.org/wiki/index.php/Part:BBa_J61100 BBa_J61100] and [http://partsregistry.org/wiki/index.php/Part:BBa_J61101 BBa_J61101]. | [https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for the insertion of a RBS based on the sequence of [http://partsregistry.org/wiki/index.php/Part:BBa_J61100 BBa_J61100] and [http://partsregistry.org/wiki/index.php/Part:BBa_J61101 BBa_J61101]. | ||
Revision as of 10:00, 14 September 2012
Contents |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB162]
Primers for the construction of a BioBrick containing the small subunit B1 of the tripartite tricarboxylate transporter family.
tctB162 BioBrick
5' BioBrick Annealing 3' fwd: gtttcttcgaattcgcggccgcttctagatgaaaatcaagagccaaaaagactttttctc rev: gtttcttcctgcagcggccgctactagtattagccagcgatgaagctaggcc
tctB162 Pst1
Primers for the elimination of a Pst1 site inside the tctB162 BioBrick.
5' 3' fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC rev: GGCCACACGGGGAACTGGAGATTCAGCGCC
tctB162 RBS
Primers for the insertion of a RBS based on the sequence of [http://partsregistry.org/wiki/index.php/Part:BBa_J61100 BBa_J61100] and [http://partsregistry.org/wiki/index.php/Part:BBa_J61101 BBa_J61101].
5' 3' fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAAAGAGGGGACAAtactag atgaaaatcaagagccaaaaagactttttctc rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttagccagcgatgaagctaggcc
