Team:TU Darmstadt/Materials/SOB

From 2012.igem.org

Revision as of 23:48, 9 September 2012 by Adrian E (Talk | contribs)

SOB

The SOB medium is used for growth of bacteria in preparation of chemically competent cells.

Ingredients

  • 0.5% (w/v) yeast extract
  • 2% (w/v) tryptone
  • 10 mM NaCl
  • 2.5 mM KCl
  • 20 mM MgSO4

For 1 L:

  • 0.186 g KCl
  • 2.4 g MgSO4
  • 0.584 g NaCl
  • 20 g tryptone
  • 5 g yeast extract

Adjust to pH 7.5 prior to use. This requires approximately 5 mL of 5M NaOH per liter.