Team:TU Darmstadt/Materials/EcoRI

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  5' GAATTC --> 5'---G    AATTC---3'
  5' GAATTC --> 5'---G    AATTC---3'
  3' CTTAAG --> 3'---CTTAA    G---5'
  3' CTTAAG --> 3'---CTTAA    G---5'
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'''Buffer:''' NEBuffer EcoRI
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'''Buffer:''' [https://2012.igem.org/wiki/index.php?title=Team:TU_Darmstadt/Materials/NEB_buffers&action=submit#NEBuffer_EcoRI NEBuffer EcoRI]
   
   
EcoRI can exhibit star activity depending on the conditions present in the reaction and needs at least 1bp overhang on its cutting site.<sup>[2]</sup>
EcoRI can exhibit star activity depending on the conditions present in the reaction and needs at least 1bp overhang on its cutting site.<sup>[2]</sup>
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=== Supplier ===
=== Supplier ===
* NEB http://www.neb.com/nebecomm/products/productR3101.asp
* NEB http://www.neb.com/nebecomm/products/productR3101.asp

Latest revision as of 20:37, 26 September 2012

Contents

EcoRI

About

EcoRI is a endonucleasic restriction enzyme isolated from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli RY13]. It creates stick ends with 5' end overhang.

Working conditions

The recognition motive of EcoRI is:[1]

5' GAATTC --> 5'---G     AATTC---3'
3' CTTAAG --> 3'---CTTAA     G---5'

Buffer: NEBuffer EcoRI

EcoRI can exhibit star activity depending on the conditions present in the reaction and needs at least 1bp overhang on its cutting site.[2]

Supplier

  • NEB http://www.neb.com/nebecomm/products/productR3101.asp

References

[1] http://en.wikipedia.org/wiki/EcoRI

[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp