Team:TU Darmstadt/Materials/Arabinose

From 2012.igem.org

Revision as of 21:01, 26 September 2012 by Vali (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Contents

Arabinose

About

Arabinose is an aldopentose that is used as an inducer for PBAD (L-Ara).[1]

Application

For a 50mL stock:

  1. Combine:
    • 10 g Arabinose (20% w/v)
    • ad. 50 mL dist. water
  2. sterile filtration
  3. store at room temperature

References

[1] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30