Team:TU-Eindhoven/Protocols

From 2012.igem.org

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<h3>Yeast transformation</h3>
<h3>Yeast transformation</h3>
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<p>The principal protocol for yeast transformation was <span class="red">Gietz and Schiestl </span>(2007)<html><a href="#ref_gietz_2007" name="text_gietz_2007"><sup>[1]</sup></a></html>. It promised high transformation efficiency, however, in combination with our yeast the <span class="red">yield</span> was a factor thousand <span class="red">lower </span> than expected. The solution was to <span class="red">increase the amount of DNA</span>used for transformation up to a whopping 3 ug and to work with freshly cultured yeast in mid-log phase instead of vials of frozen competent cells. For more details, please refer to the <html><a href="https://static.igem.org/mediawiki/2012/d/da/Yeast_transformation.pdf" target="_blank">modified yeast transformation protocol</a></html>.</p>
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<p>The principal protocol for yeast transformation was Gietz and Schiestl (2007)<html><a href="#ref_gietz_2007" name="text_gietz_2007"><sup>[1]</sup></a></html>. It promised high transformation efficiency, however, in combination with our yeast the <span class="red">yield</span> was a factor thousand <span class="red">lower </span> than expected. The solution was to <span class="red">increase the amount of DNA</span> used for transformation up to a whopping 3 ug and to work with freshly cultured yeast in mid-log phase instead of vials of frozen competent cells. For more details, please refer to the <html><a href="https://static.igem.org/mediawiki/2012/d/da/Yeast_transformation.pdf" target="_blank">modified yeast transformation protocol</a></html>.</p>

Revision as of 22:12, 26 September 2012