Team:TU-Eindhoven/Protocols

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<h3>Yeast transformation</h3>
<h3>Yeast transformation</h3>
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<p>The principal protocol for yeast transformation was Gietz and Schiestl (2007)<html><a href="#ref_gietz_2007" name="text_gietz_2007"><sup>[1]</sup></a></html>. It promised high transformation efficiency, however, in combination with our yeast the yield was a factor thousand lower than expected. The solution was to increase the amount of DNA used for transformation up to a whopping 3 ug and to work with freshly cultured yeast in mid-log phase instead of vials of frozen competent cells.</p>
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<p>The principal protocol for yeast transformation was Gietz and Schiestl (2007)<html><a href="#ref_gietz_2007" name="text_gietz_2007"><sup>[1]</sup></a></html>. It promised high transformation efficiency, however, in combination with our yeast the yield was a factor thousand lower than expected. The solution was to increase the amount of DNA used for transformation up to a whopping 3 ug and to work with freshly cultured yeast in mid-log phase instead of vials of frozen competent cells. For more details, please refer to the [[File:yeast_transformation.pdf|modified yeast transformation protocol]].</p>
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<p>For more details, please refer to the [[File:yeast_transformation.pdf|modified yeast transformation protocol]].</p>
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Revision as of 13:58, 25 September 2012