Team:TU-Eindhoven/LEC/Lab

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Expected very soon!

Plasmid construction

The following plasmids have been constructed:

• pYES2/R-GECO, pYES2/G-GECO , pYES2/B-GECO
• pYES3/R-GECO, pYES3/G-GECO , pYES3/B-GECO
• pET_28a/R-GECO, pET_28a/G-GECO , pET_28a/B-GECO
• pSB1C3/R-GECO, pSB1C3/G-GECO, pSB1C3/B-GECO

The following plasmids were received from addgene.org:

• CMV/R-GECO, CMV/G-GECO , CMV/B-GECO

These plasmids were kindly donated by H. Iida & K. Iida:

• pBCT/CCH1H, pBCT/CCH1H-HA4, pBCT/CCH1H-EGFP, YCpT-MID1, YCpT-MID1-EGFP

GECO protein expression and isolation

The three GECOs have been expressed in E. coli BL21 to yield a large amount of the proteins for characterization. Cells were cultured in large Erlenmeyer flasks. The results were visually impressive: After lysis the flasks turned vibrantly red and green respectively. The blue GECO however looked much the same as the green GECO.

TODO: COLUMN FILTRATION

Yeast transformation

Device tests

Spectrophotometry

References