Team:TU-Eindhoven/LEC/Accomplishments

From 2012.igem.org

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<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Register the team, have a great summer and plan to have fun at the Regional Jamboree</h3>  
<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Register the team, have a great summer and plan to have fun at the Regional Jamboree</h3>  
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As can also be seen at our [[Team:TU-Eindhoven/Photos|photos]] we had a great summer!</td></tr>
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As can also be seen at our [[Team:TU-Eindhoven/Photos|photos]] we had a <span class="lightblue">great summer!</span></td></tr>
<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Complete the judging form</h3>  
<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Complete the judging form</h3>  
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<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Create and present a wiki, poster and talk</h3>  
<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Create and present a wiki, poster and talk</h3>  
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As you can see our wiki is done and of course we are going to present our poster and talk at the Regional Jamboree.</td></tr>
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As you can see our wiki is done and of course we are going to present our poster and talk at the <span class="lightblue">Regional Jamboree.</span></td></tr>
<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Create and document a new BioBrick</h3>  
<tr valign="top"><td>[[File:Bronze medal.png|50px|left|link=]]</td><td><h3>Create and document a new BioBrick</h3>  
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Created new Ca<sup>2+</sup> sensor BioBricks. See [[Team:TU-Eindhoven/LEC/BioBricks|our BioBricks]].</td></tr>
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<span class="lightblue">Created</span> new Ca<sup>2+</sup> sensor BioBricks. See [[Team:TU-Eindhoven/LEC/BioBricks|our BioBricks]].</td></tr>
<tr valign="top"><td>[[File:Silver medal-blue.png|50px|left|link=]] </td><td>
<tr valign="top"><td>[[File:Silver medal-blue.png|50px|left|link=]] </td><td>
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<tr valign="top"><td>[[File:Gold medal.png|50px|left|link=]]</td><td>
<tr valign="top"><td>[[File:Gold medal.png|50px|left|link=]]</td><td>
<h3>Discovery Festival</h3>
<h3>Discovery Festival</h3>
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We organized an iGEM workshop on the Discovery Festival in the Netherlands in collaboration with 3 other Dutch iGEM teams: Amsterdam, Groningen and Wageningen_UR. To cooperate in this large project, the 4 teams met a couple of times on different locations in the Netherlands. Besides these meetings, we discussed a lot during several Skype meetings. We will take our visitors on a tour in our provisional laboratory, where they will learn about the process of genetic modification. The festival will take place in 3 cities, Amsterdam, Rotterdam and Eindhoven, and over 5000 visitors are expected. Hopefully, this will inspire other teams in upcoming years to combine forces in reaching out to the public. More information can be found on [[Team:TU-Eindhoven/Human_Practices|the public outreach page]].</td></tr>
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We organized an iGEM workshop on the Discovery Festival in the Netherlands in collaboration with 3 other Dutch iGEM teams: <span class="lightblue">Amsterdam, Groningen and Wageningen_UR.</span> To cooperate in this large project, the 4 teams met a couple of times on different locations in the Netherlands. Besides these meetings, we discussed a lot during several Skype meetings. We will take our visitors on a tour in our provisional laboratory, where they will learn about the process of genetic modification. The festival will take place in 3 cities, Amsterdam, Rotterdam and Eindhoven, and over 5000 visitors are expected. Hopefully, this will <span class="lightblue">inspire</span> other teams in upcoming years to combine forces in reaching out to the public. More information can be found on [[Team:TU-Eindhoven/Human_Practices|the public outreach page]].</td></tr>
<tr valign="top"><td>[[File:Gold medal.png|50px|left|link=]]</td><td>
<tr valign="top"><td>[[File:Gold medal.png|50px|left|link=]]</td><td>
<h3>Collaborations</h3>
<h3>Collaborations</h3>
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We met all Dutch iGEM teams during a symposium in the first week of the summer break. Furthermore, we posted two offers on the NTNU-Trondheim Matchmaker, we helped the teams of Queens_Canada and Trieste with their computational models and we came up with 'tips and tricks' for new iGEM teams for the team of Cinvestav. Just before the Regional Jamboree, the Dutch teams will meet again during a symposium in Eindhoven, where every team can rehearse their final presentation. Some German and Belgian teams were also invited to this symposium. See our <html><a href="https://2012.igem.org/Team:TU-Eindhoven/Collaborations" target="_blank">collaborations page.</a></html></td></tr>
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We met all Dutch iGEM teams during a <span class="lightblue">symposium</span> in the first week of the summer break. Furthermore, we posted <span class="lightblue">two offers on the NTNU-Trondheim Matchmaker,</span> we helped the teams of Queens_Canada and Trieste with their computational models and we came up with 'tips and tricks' for new iGEM teams for the team of Cinvestav. Just before the Regional Jamboree, the Dutch teams will meet again during <span class="lightblue">a symposium in Eindhoven</span>, where every team can rehearse their final presentation. Some German and Belgian teams were also invited to this symposium. See our <html><a href="https://2012.igem.org/Team:TU-Eindhoven/Collaborations" target="_blank">collaborations page.</a></html></td></tr>
<tr valign="top"><td class="star">[[File:Star.png|50px|left|link=]]</td><td>
<tr valign="top"><td class="star">[[File:Star.png|50px|left|link=]]</td><td>
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<tr valign="top"><td class="star">[[File:Star.png|50px|left|link=]]</td><td>
<tr valign="top"><td class="star">[[File:Star.png|50px|left|link=]]</td><td>
<h3>Model</h3>
<h3>Model</h3>
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A sensitivity analysis is performed to a <html><a href="https://2012.igem.org/Team:TU-Eindhoven/LEC/ModellingFirst" target="_blank">basic calcium model</a></html>, first used by the iGEM team of Valencia in 2009, so we found out which parameters are most sensitive. Furthermore, we made an <html><a href="https://2012.igem.org/Team:TU-Eindhoven/Modeling" target="_blank">extended calcium model</a></html>, in which the kinetics of the calcium homeostasis, voltage-gated calcium channels and GECO proteins are taken into account. By combining these three characteristics, we created a model for a completely new system.</td></tr>
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A sensitivity analysis is performed to a <html><a href="https://2012.igem.org/Team:TU-Eindhoven/LEC/ModellingFirst" target="_blank">basic calcium model</a></html>, first used by the iGEM team of Valencia in 2009, so we found out which <span class="lightblue">parameters</span> are <span class="lightblue">most sensitive</span>. Furthermore, we made an <html><a href="https://2012.igem.org/Team:TU-Eindhoven/Modeling" target="_blank">extended calcium model</a></html>, in which the kinetics of the calcium homeostasis, voltage-gated calcium channels and GECO proteins are taken into account. By combining these three characteristics, we created a model for <span class="lightblue">a completely new system.</span></td></tr>
<tr valign="top"><td class="star">[[File:Star.png|50px|left|link=]]</td><td>
<tr valign="top"><td class="star">[[File:Star.png|50px|left|link=]]</td><td>
<h3>Yeast cells</h3>
<h3>Yeast cells</h3>
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In the lab, we created three kinds of <html><a href=" https://2012.igem.org/Team:TU-Eindhoven/LEC/Lab" target="_blank">yeast cells</a></html>, containing both the channel proteins CCH1 and MID1 and a red, green or blue GECO calcium sensor.</td></tr>
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In the lab, we <span class="lightblue">created</span> three kinds of <html><a href=" https://2012.igem.org/Team:TU-Eindhoven/LEC/Lab" target="_blank">yeast cells</a></html>, containing both the channel proteins CCH1 and MID1 and a red, green or blue GECO calcium sensor.</td></tr>
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Revision as of 21:31, 26 September 2012