Membrane Achor 1 (BBa_K771001) Membrane Achor 2 (BBa_K771002) Membrane Achor 3 (BBa_K771003) Membrane Achor 4 (BBa_K771004) Membrane Achor 5 (BBa_K771005) Membrane Achor 6 (BBa_K771006)
Membrane Achor 2 without MS2(BBa_K771007) Membrane Achor 3 (BBa_K771003) Membrane Achor 4 (BBa_K771004) Membrane Achor 5 without VVD(BBa_K771008)
Membrane Achor 0 (BBa_K771000)
ssDsbA-β-Bla-Lgt-GFP (BBa_K77401)
To justify the localization ability of fusion membrane proteins mentioned above, we constructed a novel fusion protein called BlaLG. β-lactamase is fused to the N-terminus of Lgt and GFP is fused to the C-terminus of Lgt. The fusion protein in under control of araBAD promoter (Fig.3). split EGFP 1 with Membrane Achor 2 without MS2 (BBa_K771407) split EGFP 2 with Membrane Achor 3 (BBa_K771403) split EGFP 1 with Membrane Achor 4 (BBa_K771404) split EGFP 2 with Membrane Anchor 5(without VVD) Membrane Achor 5 without VVD(BBa_K771405)