Fatty Acid

FAS

Fatty acid biosynthesis pathways in E.coli, showing the role of FabG, FabZ, FabI and TesA.

Catalytic cycle of the E. coli fatty acid synthase. (A) Initiation: In the presence of holo-ACP, NADPH and NADH, acetyl-CoA and malonyl-CoA undergo condensation and subsequent reduction to form butyryl-ACP. These reactions are catalyzed by the malonyl-CoA:ACP transacylase FabD, the ketosynthase FabH, the NADPH-dependent ketoreductase FabG, either the dual-function dehydratase/isomerase FabA or the monofunctional dehydra- tase FabZ, and the NADH-dependent enoyl reductase FabI. (B) Elongation: Butyryl-ACP is extended via 5–7 rounds of analogous reactions to produce a C14 to C18-ACP that is either fully saturated or monounsaturated. These extension cycles are catalyzed by either the ketosynthase FabB or FabF in collaboration with FabD, FabG, FabA or FabZ, and FabI. (C) Termination: The full-length fatty acid is released from the corresponding fatty acyl-ACP via hydrolysis by the thioesterase (TesA).

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771301 TesA (BBa_K771301)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771302 FabG (BBa_K771302)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771303 FabI (BBa_K771303)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771304 FabZ (BBa_K771304)]

Fatty Acid Enzyme with Membrane Anchor System

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771305 TesA with Membrane Anchor 2 (without MS2) (BBa_K771305)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771306 FabG with Membrane Anchor 3 (BBa_K771306)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771307 FabZ with Membrane Anchor 4 (BBa_K771307)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771308 FabI with Membrane Anchor 4(without VVD ) (BBa_K771308)]