Team:Osaka/week7

From 2012.igem.org

(Difference between revisions)
Line 17: Line 17:
# Ligation
# Ligation
#* 160(K):157(upstream)+158(downstream)+1-5A(vector)
#* 160(K):157(upstream)+158(downstream)+1-5A(vector)
-
# Transformation of ligation products
+
# Transformation of ligation products into DH5α <i>E.coli</i>
# Transfer to liquid culture(146,148)
# Transfer to liquid culture(146,148)
# Tolerance assay of 144,145(MMC)
# Tolerance assay of 144,145(MMC)
Line 27: Line 27:
# Ligation
# Ligation
#* 160(K):157(upstream)+158(downstream)+1-5A(vector)
#* 160(K):157(upstream)+158(downstream)+1-5A(vector)
-
# Transformation of ligation products
+
# Transformation of ligation products into DH5α <i>E.coli</i>
-
# Tolerance assay of 146,148(MMC)
+
# Tolerance assay of 146,148(MMC)  
-
 
+
==September 12 (Wed)==
==September 12 (Wed)==
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#* 161(C):1-9N(upstream)+160(downstream)+1-3A(vector)
#* 161(C):1-9N(upstream)+160(downstream)+1-3A(vector)
#* 162(C):159(upstream)+160(downstream)+1-3A(vector)
#* 162(C):159(upstream)+160(downstream)+1-3A(vector)
 +
# Transformation of ligation products into DH5α <i>E.coli</i>
==September 13 (Thu)==
==September 13 (Thu)==
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# Sequencing(157,158,159,160)
# Sequencing(157,158,159,160)
# Tolerance assay of 146,148
# Tolerance assay of 146,148
-
# Transformation of ligation products:161
+
# Transformation of ligation products into DH5α <i>E.coli</i>:161
# Transfer to liquid culture(144~148,162)
# Transfer to liquid culture(144~148,162)

Revision as of 04:49, 15 September 2012


September 9 (Sun)

  1. Miniprep of yesterday's culture(150,151,152,159,160)
  2. Restriction digests of minipreppped parts
  3. Electrophoresis
  4. Tolerance assay of 144,147(MMC)
  5. Transfer to liquid culture(144,145,160)


September 10 (Mon)

  1. Miniprep of yesterday's culture(160,1-9N)
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Preparation of LB agar plates(Amp)
  5. Ligation
    • 160(K):157(upstream)+158(downstream)+1-5A(vector)
  6. Transformation of ligation products into DH5α E.coli
  7. Transfer to liquid culture(146,148)
  8. Tolerance assay of 144,145(MMC)


September 11 (Tue)

  1. Restriction digests(157,158,159,160,1-9N)
  2. Gel electrophoresis of digests
  3. Ligation
    • 160(K):157(upstream)+158(downstream)+1-5A(vector)
  4. Transformation of ligation products into DH5α E.coli
  5. Tolerance assay of 146,148(MMC)

September 12 (Wed)

  1. Miniprep of yesterday's culture:160
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Tolerance assay
  5. Ligation
    • 161(C):1-9N(upstream)+160(downstream)+1-3A(vector)
    • 162(C):159(upstream)+160(downstream)+1-3A(vector)
  6. Transformation of ligation products into DH5α E.coli

September 13 (Thu)

  1. Colony check
    • 161:Transformed cells didnot grow
  2. Sequencing(157,158,159,160)
  3. Tolerance assay of 146,148
  4. Transformation of ligation products into DH5α E.coli:161
  5. Transfer to liquid culture(144~148,162)


September 14 (Fri)

  1. Miniprep of yesterday's culture:162
  2. Restriction digests of minipreppped parts
  3. Tolerance assay (Preliminary test of hydrogen peroxide)
  4. Gel electrophoresis of digests
  5. Transfer to liquid culture:149,152,161

September 15 (Sat)

  1. Miniprep of yesterday's culture:161
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Gel extraction :161,162
  5. Ligation
    • 163(C):161(upstream)+151(downstream)+1-3A(vector)
    • 164(C):161(upstream)+152(downstream)+1-3A(vector)
    • 165(C):162(upstream)+151(downstream)+1-3A(vector)
    • 166(C):162(upstream)+152(downstream)+1-3A(vector)
  6. Transformation of ligation products into DH5α E.coli
  7. Tolerance assay (Hydrogen peroxide)
  8. Transfer to liquid culture:


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