Team:Osaka/week2

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August 5 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transfer to liquid culture
    • (Rosetta)143~146,149,152
    • (DH5α)150,Empty vector(A)

August 6 (Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transformaton of Registry parts into DH5α E.coli(See Table 2).
Table2
IDPart NameResistanceDescription
1-2D<bbpart>BBa_J52008</bbpart>A,K Renilla Luciferase
1-10H<bbpart>BBa_I712019</bbpart>A,K Firefly Luciferase from Photinus pyralis
1-22A<bbpart>BBa_J23118</bbpart>A Constitutive promoter family member


August 7 (Tue)

August 8 (Wed)

August 9 (Thu)

August 10 (Fri)

August 11 (Sat)

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