Team:Osaka/week1

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August 1 (Wed)

  1. Preparation of LB agar plates (Amp)
  2. Restriction digests of parts (See Table 1)
Table 1
numberPart NameResistanceDescription
124<bbpart>BBa_K602015</bbpart>C D. radiodurans<i> PprI+PprA expression device
125<bbpart>BBa_K602016</bbpart>C <i>D. radiodurans<i> PprI+RecA expression device
126<bbpart>BBa_K602017</bbpart>C <i>D. radiodurans<i> PprA+RecA expression device
127<bbpart>BBa_K602018</bbpart>C <i>D. radiodurans<i> PprI+PprM expression device
128<bbpart>BBa_K602019</bbpart>C <i>D. radiodurans<i> PprA+PprM expression device
129<bbpart>BBa_K602020</bbpart>C <i>D. radiodurans<i> PprM+RecA expression device


  1. Ligation
    • 143(A): 124+1-1G(vector)
    • 144(A): 125+1-1G(vector)
    • 145(A): 126+1-1G(vector)
    • 146(A): 127+1-1G(vector)
    • 147(A): 128+1-1G(vector)
    • 148(A): 129+1-1G(vector)
    • 149(A): 138+1-1G(vector)
    • 150(A): 139+1-1G(vector)
    • 151(A): 140+1-1G(vector)
    • 152(A): 141+1-1G(vector)
  2. Transformation of ligation products

August 2 (Thu)

August 3 (Fri)

August 4 (Fri)

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