Team:Osaka/Project

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{{Osaka}}
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Osaka|Home]]
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!align="center"|[[Team:Osaka/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Osaka Official Team Profile]
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!align="center"|[[Team:Osaka/Project|Project]]
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!align="center"|[[Team:Osaka/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Osaka/Modeling|Modeling]]
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!align="center"|[[Team:Osaka/Notebook|Notebook]]
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!align="center"|[[Team:Osaka/Safety|Safety]]
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!align="center"|[[Team:Osaka/Attributions|Attributions]]
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== '''Overall project''' ==
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Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)
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== Project Details==
== Project Details==
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=== DNA damage tolerance===
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==== <i>D. radiodurans</i> ====
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[[File:deinococcus.jpg|200px|right|''D.radiodurans'']]
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The bacterium ''Deinococcus radiodurans'' shows remarkable resistance to a range of DNA damage caused by ionizing radiation, desiccation, UV radiation, oxidizing agents, and electrophilic mutagens. It is an aerobically-growing bacterium that is most famous for its extreme resistance to ionizing radiation; it not only can survive acute exposures to gamma radiation that exceed 15,000 Gy, but it can also grow continuously in the presence of chronic radiation (60 Gy/hour)  without any effect on its growth rate or ability to express cloned genes. For comparison, <i>E. coli</i> can withstand up to 200 Gy, and an acute exposure of just 5-10 Gy is lethal to a human being.</p>
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=== Part 2 ===
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<p>We explored various genes from <i>D. radiodurans</i>, implicated in its remarkable DNA damage resistance. By BioBricking selected genes and transforming them into <i>E. coli</i>, we hoped to confer additional DNA damage tolerance to the host cells.</p>
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Revision as of 05:51, 17 August 2012


Project Details

DNA damage tolerance

D. radiodurans

D.radiodurans

The bacterium Deinococcus radiodurans shows remarkable resistance to a range of DNA damage caused by ionizing radiation, desiccation, UV radiation, oxidizing agents, and electrophilic mutagens. It is an aerobically-growing bacterium that is most famous for its extreme resistance to ionizing radiation; it not only can survive acute exposures to gamma radiation that exceed 15,000 Gy, but it can also grow continuously in the presence of chronic radiation (60 Gy/hour) without any effect on its growth rate or ability to express cloned genes. For comparison, E. coli can withstand up to 200 Gy, and an acute exposure of just 5-10 Gy is lethal to a human being.

We explored various genes from D. radiodurans, implicated in its remarkable DNA damage resistance. By BioBricking selected genes and transforming them into E. coli, we hoped to confer additional DNA damage tolerance to the host cells.

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