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Team:Evry/auxin production
From 2012.igem.org
Auxin production
Overview
The 2011 Imperial College iGEM (https://2011.igem.org/Team:Imperial_College_London) project showed that the plant hormone auxin or indole-3-acetic acid (IAA) can be synthetized in E.coli through a two-step pathway from tryptophan. In their work, the genes encoding the auxin biosynthesis pathway originally coming from Pseudomonas savastanoi were expressed in Escherichia coli. In our project we aim to express this auxin biosynthesis pathway in Xenopus tropicalis . First, we plan to reengineer the auxin biosynthesis pathway in the tadpole with a ubiquitous and constitutive promoter. Once the system is functional, we intend to put the system under the control of tissue-specific promoters.
Hypothesis
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Model description
Equations
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Geometry
Limit conditions
Calibration
Results
Conclusion
References
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