Team:Cornell/testing/project/wetlab/5
From 2012.igem.org
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- | <h3>Proteolysis tag as means to | + | <h3>Proteolysis tag as means to lessen the effects of leaky expression</h3> |
By fusing a proteolysis tag to MtrB, MtrB would be degraded by proteasomes at a higher rate. This would allow us to tune the degradation of MtrB such that protein concentrations at non-induced levels are insufficient for complexing with other components of the Mtr pathway and hence, at producing current. By decreasing the basal current levels of our strains, the dynamic range of our biosensing platform would be increased. | By fusing a proteolysis tag to MtrB, MtrB would be degraded by proteasomes at a higher rate. This would allow us to tune the degradation of MtrB such that protein concentrations at non-induced levels are insufficient for complexing with other components of the Mtr pathway and hence, at producing current. By decreasing the basal current levels of our strains, the dynamic range of our biosensing platform would be increased. |
Revision as of 03:52, 4 October 2012
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Wet Lab
- Overview
- Chassis
- DNA Assembly
- Testing & Results
- Future Work
- Animation