Team:Cornell/testing/project

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We linked arsenic- and naphthalene-sensing systems to the electrode-reducing capability of <i>Shewanella oneidensis </i>MR-1 to synthesize an electrical reporter system. Read more about the underlying biology, as well as our approaches to molecular cloning and characterization by clicking the links below.  
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<h3 class="centered" style="margin:0px"> We have developed a novel biosensor intended for continuous monitoring of water quality in areas affected by oil and gas extraction.</h3>
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Canadian oil sands are a vast oil reserve that, given rising prices of petroleum, are an attractive alternative to traditional sources of crude oil. However, there are numerous public health and environmental concerns regarding the oil sands extraction process. One environmental concern is the contamination of Canadian watersheds by seepage from tailings ponds. To better monitor this issue, we have engineered a novel biosensing platform with the electroactive bacterial species Shewanella oneidensis MR-1, which has the unique capability to directly transfer electrons to solid-state electrodes. We exploit this feature by genetically manipulating S. oneidensis MR-1 to upregulate its metal-reduction capacity in the presence of analyte to generate direct current output in a whole-cell biosensor. Our goal is to develop a fully autonomous electrochemical biosensor that complements the current oil sands monitoring system by providing real-time data over extended periods of time. Furthermore, our device will circumvent the costs and complications of producing and maintaining photodiode circuits used for data acquisition in bioluminescent reporter systems by instead producing a direct electrical output. While our platform is adaptable to sensing a wide range of analytes, we will initially focus on arsenic-containing compounds and naphthalene,a polycyclic aromatic hydrocarbon (PAH) – known contaminants of oil sands tailings ponds. We believe that our biosensor will be a valuable tool for remote,continuous, and long-term monitoring of pollutants in rivers and key watersheds.
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<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/1">What are the Oil Sands?</a>
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</li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/2">How is oil extracted from Oil Sands?</a>
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</li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/3">Environmental Concerns</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/4">Health Effects from Water Contaminated with Arsenic
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and Naphthalene</a>
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</ul>
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<b>We built a physical device to house our bioelectrochemical reactor and designed custom electronics to wirelessly transmit measured current to a central server. Read more about the mechanical and electrical engineering, computer science, and applied mathematics that went into our project by clicking the links below. </b>
 
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<h4 class="centered">Human Practices</h4>
 
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We adopted a novel approach to holistically assess the impacts and risks of our project. Read more about our <b> Comprehensive Environmental Assessment</b>, as well as our other human practices applications, by clicking the links below.
 
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/1">Overview</a>
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<h4 class="centered">Wet Lab</h4>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/2">Chassis</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/1">Overview</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/3">DNA Assembly</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4">Testing and Results</a>
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DNA Assembly
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/5">Future Work</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/3/1">Arsenic Reporter</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/1">Overview</a>
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Testing & Results
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/2">Specifications</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/3">Design</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/4">Modeling</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/2">Fluorescence</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/5">Assembly</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/3">qPCR</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/6">Testing and Results</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/4">Western</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/7">Future Work</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/5">Artificial River Media Growth Assays</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/6">Naphthalene Growth Assays</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/economic">Comprehensive Environmental Analysis</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/5">Future work</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/fracking">Application: Oil Sands</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/6">Animation</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/watershed">Application: Cayuga Watershed</a>
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<h4 class="centered">Dry Lab</h4>
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<ul>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/1">Overview</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/2">Specifications</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/3">Design</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/4">Modeling</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/6">Animation</a>
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</li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/5">Gallery</a>
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</li>
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</ul>
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</div>
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<div class="row">
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<div class="twelve columns">
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<h4 class="centered">Human Practices</h4>
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<ul>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/1">Comprehensive Environmental Assessment</a>
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</li>
<li>
<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/safety">Safety Overview</a>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/5">Bioethics</a>
</li>
</li>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/2">Application: Oil Sands</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/3">Application: Cayuga Watershed</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/4">Safety Overview</a>
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</li>
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</ul>
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Latest revision as of 07:02, 17 October 2012

We have developed a novel biosensor intended for continuous monitoring of water quality in areas affected by oil and gas extraction.

Canadian oil sands are a vast oil reserve that, given rising prices of petroleum, are an attractive alternative to traditional sources of crude oil. However, there are numerous public health and environmental concerns regarding the oil sands extraction process. One environmental concern is the contamination of Canadian watersheds by seepage from tailings ponds. To better monitor this issue, we have engineered a novel biosensing platform with the electroactive bacterial species Shewanella oneidensis MR-1, which has the unique capability to directly transfer electrons to solid-state electrodes. We exploit this feature by genetically manipulating S. oneidensis MR-1 to upregulate its metal-reduction capacity in the presence of analyte to generate direct current output in a whole-cell biosensor. Our goal is to develop a fully autonomous electrochemical biosensor that complements the current oil sands monitoring system by providing real-time data over extended periods of time. Furthermore, our device will circumvent the costs and complications of producing and maintaining photodiode circuits used for data acquisition in bioluminescent reporter systems by instead producing a direct electrical output. While our platform is adaptable to sensing a wide range of analytes, we will initially focus on arsenic-containing compounds and naphthalene,a polycyclic aromatic hydrocarbon (PAH) – known contaminants of oil sands tailings ponds. We believe that our biosensor will be a valuable tool for remote,continuous, and long-term monitoring of pollutants in rivers and key watersheds.