Team:Cornell/testing/notebook/drylab/2

From 2012.igem.org

(Difference between revisions)
 
(3 intermediate revisions not shown)
Line 13: Line 13:
</li>
</li>
<li class="divider"></li>
<li class="divider"></li>
-
<li>
+
-
<a href="https://2012.igem.org/Team:Cornell/testing/notebook/drylab/pre">Pre</a>
+
-
</li>
+
<li>
<li>
<a href="https://2012.igem.org/Team:Cornell/testing/notebook/drylab/1">Week 1</a>
<a href="https://2012.igem.org/Team:Cornell/testing/notebook/drylab/1">Week 1</a>
Line 84: Line 82:
</div>
</div>
</div>
</div>
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Sunday</h3>
+
-
It has been said that <span class="has-tip" data-width="250" title="Description of what astronomy is.">astronomy</span> is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known. <a href="#" class="technical-desc" for="#technical-desc1" style="display:block;margin-top:20px;">Details</a>
+
-
<div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc1">
+
-
<h6>Entry:</h6>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Monday</h3>
+
-
BRIEFINGS
+
-
<a href="#" class="technical-desc" for="#technical-desc7" style="display:block;margin-top:20px;">Details</a>
+
-
<div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc7">
+
-
<h6>Entry:</h6>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Tuesday</h3>
+
-
BRIEFINGS
+
-
<a href="#" class="technical-desc" for="#technical-desc2" style="display:block;margin-top:20px;">Details</a>
+
-
<div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc2">
+
-
<h6>Entry:</h6>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
<div class="row">
<div class="row">
<div class="nine columns">
<div class="nine columns">
Line 143: Line 103:
</div>
</div>
<div class="three columns">
<div class="three columns">
-
<img src="http://placehold.it/250x250/321852">
+
<img src="https://static.igem.org/mediawiki/2012/e/e1/Smile_square.jpg">
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Thursday</h3>
+
-
BRIEFINGS
+
-
<a href="#" class="technical-desc" for="#technical-desc4" style="display:block;margin-top:20px;">Details</a>
+
-
<div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc4">
+
-
<h6>Entry:</h6>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Friday</h3>
+
-
BRIEFINGS
+
-
<a href="#" class="technical-desc" for="#technical-desc5" style="display:block;margin-top:20px;">Details</a>
+
-
<div class="hide-me panel" style="background:white;margin-top: 20px;" id="technical-desc5">
+
-
<h6>Entry:</h6>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
</div>
</div>
</div>
</div>
 +
 +
<div class="row last-ele">
<div class="row last-ele">
<div class="nine columns">
<div class="nine columns">
Line 191: Line 125:
</div>
</div>
<div class="three columns">
<div class="three columns">
-
<img src="http://placehold.it/250x250/321852">
+
<img src="https://static.igem.org/mediawiki/2012/b/b0/06_16.jpg">
</div>
</div>
</div>
</div>
Line 212: Line 146:
</div>
</div>
</div>
</div>
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Sunday</h3>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Monday</h3>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Tuesday</h3>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
<div class="row">
<div class="row">
<div class="nine columns">
<div class="nine columns">
Line 250: Line 160:
</div>
</div>
<div class="three columns">
<div class="three columns">
-
<img src="http://placehold.it/250x250/321852">
+
<img src="https://static.igem.org/mediawiki/2012/e/e1/Smile_square.jpg">
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Thursday</h3>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Friday</h3>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
</div>
</div>
</div>
</div>
 +
 +
<div class="row last-ele">
<div class="row last-ele">
<div class="nine columns">
<div class="nine columns">
Line 281: Line 175:
</div>
</div>
<div class="three columns">
<div class="three columns">
-
<img src="http://placehold.it/250x250/321852">
+
<img src="https://static.igem.org/mediawiki/2012/b/b0/06_16.jpg">
</div>
</div>
</div>
</div>
Line 302: Line 196:
</div>
</div>
</div>
</div>
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Sunday</h3>
+
-
BRIEFINGS
+
-
<div class="panel" style="background:white;margin-top: 20px;">
+
-
<h6>Entry:</h6>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Monday</h3>
+
-
BRIEFINGS
+
-
<div class="panel" style="background:white;margin-top: 20px;">
+
-
<h6>Entry:</h6>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Tuesday</h3>
+
-
BRIEFINGS
+
-
<div class="panel" style="background:white;margin-top: 20px;">
+
-
<h6>Entry:</h6>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
<div class="row">
<div class="row">
<div class="nine columns">
<div class="nine columns">
Line 358: Line 216:
</div>
</div>
<div class="three columns">
<div class="three columns">
-
<img src="http://placehold.it/250x250/321852">
+
<img src="https://static.igem.org/mediawiki/2012/e/e1/Smile_square.jpg">
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Thursday</h3>
+
-
BRIEFINGS
+
-
<div class="panel" style="background:white;margin-top: 20px;">
+
-
<h6>Entry:</h6>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
-
</div>
+
-
</div>
+
-
<div class="row">
+
-
<div class="nine columns">
+
-
<h3>Friday</h3>
+
-
BRIEFINGS
+
-
<div class="panel" style="background:white;margin-top: 20px;">
+
-
<h6>Entry:</h6>
+
-
ALLTHEDETAILS
+
-
</div>
+
-
</div>
+
-
<div class="three columns">
+
-
<img src="http://placehold.it/250x250/321852">
+
</div>
</div>
</div>
</div>
 +
 +
<div class="row last-ele">
<div class="row last-ele">
<div class="nine columns">
<div class="nine columns">
Line 403: Line 237:
</div>
</div>
<div class="three columns">
<div class="three columns">
-
<img src="http://placehold.it/250x250/321852">
+
<img src="https://static.igem.org/mediawiki/2012/b/b0/06_16.jpg">
</div>
</div>
</div>
</div>

Latest revision as of 03:25, 4 October 2012

Progress Log
Details
Both

Week 2

  • Wednesday, June 13, 2012

    Focus: System Flow

    Discrete versus continuous: debate on food and water delivery system continues. Details
    Entry:
    Today was a huge discussion day, in which we continued our previous discussion on fluid flow through the system. Last time, we leaned toward a discrete system because it would cut energy costs due to less activity. However, Dylan told us today that discrete flow would not work because the culture needs to maintain steady state. According to the equations and graphs he drew on the whiteboard, discrete flow would not satisfy this requirement. Any deviation from steady state would add noise to the electrical output from bacteria, rendering the biosensor inaccurate.

    Still, Maneesh and Dylan tried to design a discrete system that would work. A programmable fish food dispenser would periodically release food into the bioreactor that held the bacterial culture. And a microcontroller would be used to activate the water pump. However, the issue of maintaining steady state still lingered.

    On the whiteboard, we also came up with two other designs--a passive gravity fed system and a continuous model. For continuous flow, pumps would be constantly churning food and water into the bioreactor. The disadvantages are incredibly low flow rates (meaning higher-end machines) and greatest power consumption.

    In the passive system, food and water input from above the water level would flow down into the bioreactor. This design allowed for continuous flow and least energy consumption. However, the bulk of this system would need to be strictly underwater, so we would have to look into waterproofing.

    #filtration #flow #gravity

    Saturday, June 16, 2012

    Focus: System Flow

    Decisions have been made! With flow method and battery selected, we move onto pumps. Details
    Entry:
    Maneesh and Dylan made a presentation for discrete flow. The system seems to be complicated, as it involves pressure gradients and the use of feedback loops and modifiers to generate pressure equalization. Since simplicity is a top priority, we returned to continuous flow because it would be easier to implement and still fulfill the functional requirements.

    During further discussion, we did a back-of-the-envelope calculation for a continuous flow rate of water. It came out to be 0.03 milliliters per minute, which meant we needed a very precise, slow-acting pump. For the purpose of conserving energy, Dan found some low power pumps, including a $5 Chinese manufactured pump and a $20 500 GPH (gallons per hour) bilge pump. However, more research is required to find the optimal pump. We also thought about methods for introducing food into the stream. One idea was to use a worm-gear compressor to slowly push through our system.

    Chie presented candidates for our biosensor battery. We selected a 100Ah deep cycle gel cell battery, after listening to suggestions from several professors. Lydia and others discussed meeting with ECE professors to figure out the logistics of the circuitry component. We have a rough plan for developing the potentiostat, which is vital for measuring changes in potential, or signal produced by the bacteria when they detect toxins in the water.

    #flow #pump #battery #potentiostat
  • Wednesday, June 13, 2012

    Today was a huge discussion day, in which we continued our previous discussion on fluid flow through the system. Last time, we leaned toward a discrete system because it would cut energy costs due to less activity. However, Dylan told us today that discrete flow would not work because the culture needs to maintain steady state. According to the equations and graphs he drew on the whiteboard, discrete flow would not satisfy this requirement. Any deviation from steady state would add noise to the electrical output from bacteria, rendering the biosensor inaccurate.

    Still, Maneesh and Dylan tried to design a discrete system that would work. A programmable fish food dispenser would periodically release food into the bioreactor that held the bacterial culture. And a microcontroller would be used to activate the water pump. However, the issue of maintaining steady state still lingered.

    On the whiteboard, we also came up with two other designs--a passive gravity fed system and a continuous model. For continuous flow, pumps would be constantly churning food and water into the bioreactor. The disadvantages are incredibly low flow rates (meaning higher-end machines) and greatest power consumption.

    In the passive system, food and water input from above the water level would flow down into the bioreactor. This design allowed for continuous flow and least energy consumption. However, the bulk of this system would need to be strictly underwater, so we would have to look into waterproofing.

    #filtration #flow #gravity

    Saturday, June 16, 2012

    Maneesh and Dylan made a presentation for discrete flow. The system seems to be complicated, as it involves pressure gradients and the use of feedback loops and modifiers to generate pressure equalization. Since simplicity is a top priority, we returned to continuous flow because it would be easier to implement and still fulfill the functional requirements.

    During further discussion, we did a back-of-the-envelope calculation for a continuous flow rate of water. It came out to be 0.03 milliliters per minute, which meant we needed a very precise, slow-acting pump. For the purpose of conserving energy, Dan found some low power pumps, including a $5 Chinese manufactured pump and a $20 500 GPH (gallons per hour) bilge pump. However, more research is required to find the optimal pump. We also thought about methods for introducing food into the stream. One idea was to use a worm-gear compressor to slowly push through our system.

    Chie presented candidates for our biosensor battery. We selected a 100Ah deep cycle gel cell battery, after listening to suggestions from several professors. Lydia and others discussed meeting with ECE professors to figure out the logistics of the circuitry component. We have a rough plan for developing the potentiostat, which is vital for measuring changes in potential, or signal produced by the bacteria when they detect toxins in the water.

    #flow #pump #battery #potentiostat
  • Wednesday, June 13, 2012

    Focus: System Flow

    Discrete versus continuous: debate on food and water delivery system continues.
    Entry:
    Today was a huge discussion day, in which we continued our previous discussion on fluid flow through the system. Last time, we leaned toward a discrete system because it would cut energy costs due to less activity. However, Dylan told us today that discrete flow would not work because the culture needs to maintain steady state. According to the equations and graphs he drew on the whiteboard, discrete flow would not satisfy this requirement. Any deviation from steady state would add noise to the electrical output from bacteria, rendering the biosensor inaccurate.

    Still, Maneesh and Dylan tried to design a discrete system that would work. A programmable fish food dispenser would periodically release food into the bioreactor that held the bacterial culture. And a microcontroller would be used to activate the water pump. However, the issue of maintaining steady state still lingered.

    On the whiteboard, we also came up with two other designs--a passive gravity fed system and a continuous model. For continuous flow, pumps would be constantly churning food and water into the bioreactor. The disadvantages are incredibly low flow rates (meaning higher-end machines) and greatest power consumption.

    In the passive system, food and water input from above the water level would flow down into the bioreactor. This design allowed for continuous flow and least energy consumption. However, the bulk of this system would need to be strictly underwater, so we would have to look into waterproofing.

    #filtration #flow #gravity

    Saturday, June 16, 2012

    Focus: System Flow

    Decisions have been made! With flow method and battery selected, we move onto pumps.
    Entry:
    Maneesh and Dylan made a presentation for discrete flow. The system seems to be complicated, as it involves pressure gradients and the use of feedback loops and modifiers to generate pressure equalization. Since simplicity is a top priority, we returned to continuous flow because it would be easier to implement and still fulfill the functional requirements.

    During further discussion, we did a back-of-the-envelope calculation for a continuous flow rate of water. It came out to be 0.03 milliliters per minute, which meant we needed a very precise, slow-acting pump. For the purpose of conserving energy, Dan found some low power pumps, including a $5 Chinese manufactured pump and a $20 500 GPH (gallons per hour) bilge pump. However, more research is required to find the optimal pump. We also thought about methods for introducing food into the stream. One idea was to use a worm-gear compressor to slowly push through our system.

    Chie presented candidates for our biosensor battery. We selected a 100Ah deep cycle gel cell battery, after listening to suggestions from several professors. Lydia and others discussed meeting with ECE professors to figure out the logistics of the circuitry component. We have a rough plan for developing the potentiostat, which is vital for measuring changes in potential, or signal produced by the bacteria when they detect toxins in the water.

    #flow #pump #battery #potentiostat