Team:Copenhagen/Project
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Revision as of 14:03, 23 September 2012
Why cyanobacteria?Conventional single celled model organisms, such as E.coli, are widely used in synthetic biology as reliable and well-characterized systems. However a nitrogen and carbon rich medium has to be supplied which is expensive in both economic and environmental terms. Using cyanobacteria capable of performing photosynthesis and removing waste CO2 as well as nitrogen fixating bypasses this problem and has the potential to be an important organism for future biotechnological research. We wish to genetically engineer cyanobacteria to produce biologically generated light, so-called bioluminescence. This not only has direct implications for development of sustainable and economic light sources, but also as a general proof-of-principle of the suitability of cyanobacteria for biotechnological research and production. Read more. |
iGEM Medals Requirements Bronze Team registrationComplete Judgment form. Team Wiki. Plan to present a poster and a talk at the iGEM Jamboree At least one new submitted and well-characterized standard BioBrick Part or Device. Silver
Demonstrate that at least one new BioBrick Part of your own design and construction works as expected. Gold For Gold Medal, either of the following must be checked
Improve an existing BioBrick Part or Device. |
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General designInitially the project sets with identification of native promoters from cyanobacteria responding to the day-night-cycle. Those will be spliced into an iGEM-biobrick together with the luxCDABE cassette containing the essential luciferase enzyme (and auxillary enzymes necessary for its function). The process is carried out in E.coli, and afterwards the complete plasmid is transferred to cyanobacteria (strain PCC6803). The endogenous transcription factors present in cyanobacteria should appropriately active transcription from the genetically spliced promoter thus driving transcription only during nighttime. In conjunction we will as a proof-of-principle experiment use the already existing plasmids pDawn and pDusk and BioBricks from the iGEM Registry, to, in a similar manner, create a luciferase containing plasmid, but with an artificial promoter system responding directly to light-dark conditions. It serves as a parallel control experiment. Both of these systems will subsequently be thoroughly analyzed to determine important characteristics of the system including kinetics and efficiency of the expression levels. To achieve this quantification we will collaborate with fellow Mathematics and Physics students at University Copenhagen. In this our date can be compared to simulations. As an example it be possible to put CO2 consumption by the cyanobacteria in relation to the emitted bioluminescence thus describing the degree of sustainability or ‘greenness’ of the system.
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ProtocolsCheck out what protocols we have used. See protocols here. |
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DataWe don't have any data yet, but it will come soon so visit regularly to get the latest update. See full data page. |
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ResultsAt this point there are no data submitted. Please check later. See our results. |
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Impact factors:Our project aims to create a regulated light-producing BioBrick and thus add it to the partsregistry of BioBricks, a continuously growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems (partsregistry.org ). Hence our project contributes to the expansion of the partsregistry, benefitting all researchers with interest in bioluminescence in cyanobacterias. The partsregistry is founded on an open source philosophy, a philosophy we see as a great advantage for development and improvement of the number BioBricks already existing. The addition of our BioBrick to this catalogue will perhaps encourage other groups to look at other aspects of the many functions of cyanobacterias and thus contribute to the formation of new biological systems further expanding the field of synthetic biology. |
Comments or questions to the team? Please mail us at igemcopenhagen@gmail.com |