Team:Cambridge/Lab book/Week 9

From 2012.igem.org

(Difference between revisions)
(Wednesday (22/08/12))
(Wednesday (22/08/12))
Line 33: Line 33:
*[[Team:Cambridge/Protocols/PCRcolony| Colony PCR]] for endogenous ''Bacillus subtilis'' Mg Riboswitch (both '+8 codons' and '-8 codons' versions) were re-run.
*[[Team:Cambridge/Protocols/PCRcolony| Colony PCR]] for endogenous ''Bacillus subtilis'' Mg Riboswitch (both '+8 codons' and '-8 codons' versions) were re-run.
*Gels were run for above reactions, only one band for positive control was visible.
*Gels were run for above reactions, only one band for positive control was visible.
 +
 +
 +
'''Cells cultured for Miller assay'''
 +
*''B. subtilis'' strains carrying F riboswitch and 'KO' strains cultured separately in LB and 5µg/mL Chloramphenicol at 37°C
 +
*''E.Coli'' carrying F riboswitch cultured in LB and 50mg/ml Ampicillin at 37°C
===Thursday (23/08/12)===
===Thursday (23/08/12)===

Revision as of 23:49, 22 August 2012

Week: 3 4 5 6 7 8 9

Contents

Monday (20/08/12)

Tuesday (21/08/12)

PCR for Mg Riboswitch


  • PCRs were run for pJS130.1 split vector ('+8 codons')
  • Colony PCR for endogenous Bacillus subtilis Mg Riboswitch ('+8 codons') was run
  • Gels were run for above reactions, only one band for half of the pJS130 vector was visible.

Wednesday (22/08/12)

PCR for Mg Riboswitch


  • PCRs were run for F Riboswitch from Yale's plasmid with overhangs for standard assembly.
  • Colony PCR for endogenous Bacillus subtilis Mg Riboswitch (both '+8 codons' and '-8 codons' versions) were re-run.
  • Gels were run for above reactions, only one band for positive control was visible.


Cells cultured for Miller assay

  • B. subtilis strains carrying F riboswitch and 'KO' strains cultured separately in LB and 5µg/mL Chloramphenicol at 37°C
  • E.Coli carrying F riboswitch cultured in LB and 50mg/ml Ampicillin at 37°C

Thursday (23/08/12)

Friday (24/08/12)