Team:Cambridge/Lab book/Week 9

From 2012.igem.org

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(Tuesday (21/08/12))
(Tuesday (21/08/12))
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'''[[Team:Cambridge/Protocols/PCRProtocol| PCR for Mg Riboswitch]]'''
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'''PCR for Mg Riboswitch'''
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*PCRs were run for pJS130.1 split vector ('+8 codons')
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*[[Team:Cambridge/Protocols/PCRProtocol| PCRs]] were run for pJS130.1 split vector ('+8 codons')
*[[Team:Cambridge/Protocols/PCRcolony| Colony PCR]] for endogenous ''Bacillus subtilis'' Mg Riboswitch was run
*[[Team:Cambridge/Protocols/PCRcolony| Colony PCR]] for endogenous ''Bacillus subtilis'' Mg Riboswitch was run
*Gels were run for above reactions, only one half of the pJS130 vector was amplified.
*Gels were run for above reactions, only one half of the pJS130 vector was amplified.

Revision as of 23:23, 22 August 2012

Week: 3 4 5 6 7 8 9

Contents

Monday (20/08/12)

Tuesday (21/08/12)

PCR for Mg Riboswitch


  • PCRs were run for pJS130.1 split vector ('+8 codons')
  • Colony PCR for endogenous Bacillus subtilis Mg Riboswitch was run
  • Gels were run for above reactions, only one half of the pJS130 vector was amplified.

Wednesday (22/08/12)

Thursday (23/08/12)

Friday (24/08/12)