Team:Cambridge/Lab book/Week 11
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[[File:BBPCR1.jpg|250px|left|thumb|PCR products from Wednesday's PCR. Lanes 2 - 3: Fluoride riboswitch biobrick genomic DNA. Lanes 4 - 5: MGRS construct genomic DNA (+8). Lanes 6 - 7: MGRS construct genomic DNA (-8). Lane 8: MGRS biobrick genomic DNA (+8).]] | [[File:BBPCR1.jpg|250px|left|thumb|PCR products from Wednesday's PCR. Lanes 2 - 3: Fluoride riboswitch biobrick genomic DNA. Lanes 4 - 5: MGRS construct genomic DNA (+8). Lanes 6 - 7: MGRS construct genomic DNA (-8). Lane 8: MGRS biobrick genomic DNA (+8).]] | ||
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+ | [[File:BBPCR2.jpg|250px|right|thumb|PCR products from Wednesday's PCR. Top: Lane 2: MGRS biobrick genomic DNA (+8). Lanes 3 - 4: MGRS construct backbone (expected size 9kbp) (+8). Lanes 5 - 6: MGRS construct backbone (expected size 9kbp) (-8). Lane 7: MGRS biobrick vector (+8). Lane 8: Positive control. Bottom: Lane 2: MGRS biobrick vector (+8). lane 3: Negative control.]] | ||
'''[[Team:Cambridge/Protocols/PCRProtocol|PCR of biobrick fragments and MGRS construct fragments.]]''' | '''[[Team:Cambridge/Protocols/PCRProtocol|PCR of biobrick fragments and MGRS construct fragments.]]''' |
Revision as of 15:07, 11 September 2012
Week: | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
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Contents |
Monday (03/09/12)
Tuesday (04/09/12)
Wednesday (05/09/12)
PCR of biobrick fragments and MGRS construct fragments.
- Fluoride and magnesium riboswitch genomic DNA amplified with PCR, along with vector fragments for turning the MGRS into a biobrick and for producing the magnesium riboswitch construct.
- PCR settings: Annealing temperature - 58 °C, Elongation step - 60 seconds.
- Genomic fragments worked well, but longer backbone fragments mostly failed.
Thursday (06/09/12)
Friday (07/09/12)
Saturday (08/09/12)
Gel for 9kb fragments of lux and flu construct:
File:Long frags PM 08.09.12.tif
Sunday (09/09/12)
Verification gel: