Team:British Columbia/NotebookKillSwitches

From 2012.igem.org

British Columbia - 2012.igem.org [http://ung.igem.org/Previous_iGEM_Competitions Previous iGEM Competitions]

Team Abstracts: 2011 2010 2009 2008

Parts Registry: [http://partsregistry.org/Cell_death Cell Death]

Killswitch BioBricks
Numbers Team Year Trigger What it Does Where?
<partinfo>BBa_I716462</partinfo> UC-Berkeley 2007 Arabinose promoter BamHI
<partinfo>BBa_K512002</partinfo> USC2011 IPTG, targets GFP gene CRISPR-mediated GFP plasmid degradation
<partinfo>BBa_K112808</partinfo> UC-Berkeley 2008 No PromoterT4 Lysis device Plate 3, 6G
<partinfo>BBa_K541545</partinfo> FAGEM 2011 IPTG Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
<partinfo>BBa_K593009</partinfo> METU-Ankara2011T7 Promoter ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
<partinfo>BBa_K628006</partinfo> St. Andrews 2011 pBad Protegrin-1 Kill Switch, (Antimicrobial peptide)
Strasbourg 2010 I don't know what the heck.
Tokyo Metropolitan 2011 Killer Bee e.coli?
UNIST Korea 2011 Killed by high and low temps, not if dark.
Harvard 2010 Barnase cleaves RNA!
<partinfo>BBa_I716211</partinfo> UC Berkeley 2007 No Promoter Harvard got IPTG inducible Barnase/Barnstar from Anderson Lab at UCB
Hong Kong 2010 Possible new killswitch, not implemented
<partinfo>BBa_K565001</partinfo> Valencia2011 ? 12 kb Colicin G
<partinfo>BBa_K565002</partinfo> Valencia 2011 ? 12 kb Colicin H
<partinfo>BBa_K565004</partinfo> Valencia 2011 No Promoter Microcin C51 production device (5 restriction sites)
<partinfo>BBa_K185004</partinfo> SJTU-BioX-Shanghai 2009 No Promoter RelE toxin+Double terminator
UNICAMP Brazil 2009 All sorts of kill switches, but may not all work in E. coli.
<partinfo>BBa_K284017</partinfo> UNICAMP-Brazil 2009 Lactate (Yeast) Lysozyme
<partinfo>BBa_K131000</partinfo> Calgary2008 SOS Promoter Colicin 2E3, 22A
<partinfo>BBa_K112806</partinfo> UC Berkeley 2008 T7 T4 Endolysin
<partinfo>BBa_K302035</partinfo> Newcastle 2010 Sucrose limit MazEF
<partinfo>BBa_K124014</partinfo> Brown 2008 No Promoter Mutated Holin Gene (Inconsistent sequencing)
<partinfo>BBa_K419003</partinfo> TzuChiU_Formosa 2010 pLysLysis Cassette - no antiholin
<partinfo>BBa_K317039</partinfo> Tokyo-NoKoGen 2010AHL AHL sensitive lysis cassette
<partinfo>BBa_K317040</partinfo> Tokyo-NoKoGen 2010 IPTG IPTG sensitive lysis cassette
<partinfo>BBa_K299806</partinfo> Warsaw 2010 MinC cell division inhibitor
<partinfo>BBa_K150007</partinfo> Heidelberg2008 Native Operon Colicin E1
<partinfo>BBa_K150010</partinfo> Heidelberg2008 Native Operon Colicin E9
<partinfo>BBa_K145230</partinfo> KULeuven 2008 HSL-LuxR ccdb
<partinfo>BBa_K117009</partinfo> NTU-Singapore 2008 IPTG Colicin E7
<partinfo>BBa_K117000</partinfo> NTU-Singapore 2008 Lysin (activates Colicin E7)
<partinfo>BBa_K108001</partinfo> Tsinghua 2008 Enterobacter phage lysis

Kill Switch Assay 1UBCiGEM2012.png