Team:British Columbia/NotebookKillSwitches

From 2012.igem.org

British Columbia - 2012.igem.org Previous iGEM Competitions

Team Abstracts: 2011 2010 2009 2008

Parts Registry: Cell Death

Killswitch BioBricks
Numbers Team Year Trigger What it Does Where?
BBa_I716462 UC-Berkeley 2007 Arabinose promoter BamHI
BBa_K512002 USC2011 IPTG, targets GFP gene CRISPR-mediated GFP plasmid degradation
BBa_K112808 UC-Berkeley 2008 No PromoterT4 Lysis device Plate 3, 6G
BBa_K541545 FAGEM 2011 IPTG Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
BBa_K593009 METU-Ankara2011T7 Promoter ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
BBa_K628006 St. Andrews 2011 pBad Protegrin-1 Kill Switch, (Antimicrobial peptide)
Strasbourg 2010 I don't know what the heck.
Tokyo Metropolitan 2011 Killer Bee e.coli?
UNIST Korea 2011 Killed by high and low temps, not if dark.
Harvard 2010 Barnase cleaves RNA!
BBa_I716211 UC Berkeley 2007 No Promoter Harvard got IPTG inducible Barnase/Barnstar from Anderson Lab at UCB
Hong Kong 2010 Possible new killswitch, not implemented
BBa_K565001 Valencia2011 ? 12 kb Colicin G
BBa_K565002 Valencia 2011 ? 12 kb Colicin H
BBa_K565004 Valencia 2011 No Promoter Microcin C51 production device (5 restriction sites)
BBa_K185004 SJTU-BioX-Shanghai 2009 No Promoter RelE toxin+Double terminator
UNICAMP Brazil 2009 All sorts of kill switches, but may not all work in E. coli.
BBa_K284017 UNICAMP-Brazil 2009 Lactate (Yeast) Lysozyme
BBa_K131000 Calgary2008 SOS Promoter Colicin 2E3, 22A
BBa_K112806 UC Berkeley 2008 T7 T4 Endolysin
BBa_K302035 Newcastle 2010 Sucrose limit MazEF
BBa_K124014 Brown 2008 No Promoter Mutated Holin Gene (Inconsistent sequencing)
BBa_K419003 TzuChiU_Formosa 2010 pLysLysis Cassette - no antiholin
BBa_K317039 Tokyo-NoKoGen 2010AHL AHL sensitive lysis cassette
BBa_K317040 Tokyo-NoKoGen 2010 IPTG IPTG sensitive lysis cassette
BBa_K299806 Warsaw 2010 MinC cell division inhibitor
BBa_K150007 Heidelberg2008 Native Operon Colicin E1
BBa_K150010 Heidelberg2008 Native Operon Colicin E9
BBa_K145230 KULeuven 2008 HSL-LuxR ccdb
BBa_K117009 NTU-Singapore 2008 IPTG Colicin E7
BBa_K117000 NTU-Singapore 2008 Lysin (activates Colicin E7)
BBa_K108001 Tsinghua 2008 Enterobacter phage lysis

Kill Switch Assay 1UBCiGEM2012.png