Team:British Columbia/Notebook

From 2012.igem.org

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Electrophoresed K12 with PIJ 790 plasmid, and plated them on Chlor plates with control plates to examine the plates.  
Electrophoresed K12 with PIJ 790 plasmid, and plated them on Chlor plates with control plates to examine the plates.  
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- Ruichen
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==June 9th==
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The EPI 300 did grow!
- Ruichen
- Ruichen

Revision as of 18:29, 9 June 2012

British Columbia - 2012.igem.org

Contents

June 5th

Grew cell cultures of EPI 300, BL21, and DH5α, with plasmid PIJ 790 in them, harvested, and stored at -80 freezer.

-Ruichen

June 6th

Learned how to electrophorese cells with biobricks (1-7B, 2-17F, 2-14N, 2-9B, 3-13M, 3-14E, 4-3I, 5-1A, 5-12O), and plated them on plates to grow colonies.

Tested KAN plates with control.

-Ruichen

June 7th

Retreated the plates previously spread, KAN plates worked and most of them grew into colonies or covered plates completely. (advised to spread the plates with 50µl recovered cells instead 100µl in the future for better colony identification).

Made more antibiotics of Amp (10µg/ml) and Chlor (34µg/ml), and more agar plates of (Amp and Chlor) (half bagful respectively).

Electrophoresed 1-1N, 2-2K, 1-5P, 2-21O using DH5α, and plated them.

-Ruichen


In the afternoon, we (Ruichen, Mehul, Grace) made many more K12 competent cells, which we put into storage. (The protocol is now on the wiki.) There was also talk from Jacob and John about buying $0.50 worth of gasoline/diesel from the nearest gas station to test solvent resistance of some of our cells. I left before that happened, though. (Would have been fun to see the attendant's reaction!)

- grace

June 8th

Tried to make more EPI 300 cells, though no obvious growth even after hours for the second time, and decided to leave it over night to see if there are any changes.

Electrophoresed K12 with PIJ 790 plasmid, and plated them on Chlor plates with control plates to examine the plates.

- Ruichen

June 9th

The EPI 300 did grow!

- Ruichen